The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Prion detection by an amyloid seeding assay
Open accessAbstract
Polymerization of recombinant prion protein (recPrP), which was produced in bacteria, into amyloid fibers was accompanied by the acquisition of prion infectivity. We report here that partially purified preparations of prions seed the polymerization of recPrP into amyloid as detected by a fluorescence shift in the dye Thioflavin T. Our amyloid seeding assay (ASA) detected PrP Sc , the sole component of the prion, in brain samples from humans with sporadic Creutzfeldt–Jakob disease, as well as in rodents with experimental prion disease. The ASA detected a variety of prion strains passaged in both mice and hamsters. The sensitivity of the ASA varied with strain type; for hamster Sc237 prions, the limit of detection was ≈1 fg. Some prion strains consist largely of protease-sensitive PrP Sc (sPrP Sc ), and these strains were readily detected by ASA. Our studies show that the ASA provides an alternative methodology for detecting both sPrP Sc and protease-resistant PrP Sc that does not rely on protease digestion or immunodetection.
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Cites
Common structure of soluble amyloid oligomers implies common mechanism of pathogenesis
Kayed, R., Head, E., Thompson, J. L., McIntire, T. M., Milton, S. C., Cotman, C. W., & Glabe, C. G. (2003) · Science · doi:10.1126/science.1079469 · Cited by 4,064
Protein aggregation and neurodegenerative disease
Ross, C. A., & Poirier, M. A. (2004) · Nature Medicine · doi:10.1038/nm1066 · Cited by 3,454
Cited by
Proteopathic tau seeding predicts tauopathy in vivo
Holmes, B. B., Furman, J. L., Mahan, T. E., Yamasaki, T. R., Mirbaha, H., Eades, W. C., Belaygorod, L., Cairns, N. J., Holtzman, D. M., & Diamond, M. I. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1411649111 · Cited by 696
Metadata
- Authors
- Colby, D. W., Zhang, Q., Wang, S., Groth, D., Legname, G., Riesner, D., & Prusiner, S. B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2007-12-26
- Volume
- 104
- Issue
- 52
- Pages
- 20914-20919
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2117944583
- Cited by
- 232
- References
- 2
- FWCI
- 5.15
- Type
- journal article
- Language
- en
- Open access
- green
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- Colby, D. W., Zhang, Q., Wang, S., Groth, D., Legname, G., Riesner, D., & Prusiner, S. B. (2007). Prion detection by an amyloid seeding assay. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0710152105
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