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Cited by 120Advanced MRI Techniques and ApplicationsAnatomy and Medical TechnologyFetal and Pediatric Neurological Disorders

High-resolution magnetic resonance histology of the embryonic and neonatal mouse: A 4D atlas and morphologic database

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Petiet, Alexandra E., Kaufman, Matthew H., Goddeeris, Matthew M., Brandenburg, Jeffrey, Elmore, Susan A., Johnson, G. Allan·2008·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0805747105PMC PMC2527911
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Abstract

Engineered mice play an ever-increasing role in defining connections between genotype and phenotypic expression. The potential of magnetic resonance microscopy (MRM) for morphologic phenotyping in the mouse has previously been demonstrated; however, applications have been limited by long scan times, availability of the technology, and a foundation of normative data. This article describes an integrated environment for high-resolution study of normal, transgenic, and mutant mouse models at embryonic and neonatal stages. Three-dimensional images are shown at an isotropic resolution of 19.5 μm (voxel volumes of 8 pL), acquired in 3 h at embryonic days 10.5–19.5 (10 stages) and postnatal days 0–32 (6 stages). A web-accessible atlas encompassing this data was developed, and for critical stages of embryonic development (prenatal days 14.5–18.5), >200 anatomical structures have been identified and labeled. Also, matching optical histology and analysis tools are provided to compare multiple specimens at multiple developmental stages. The utility of the approach is demonstrated in characterizing cardiac septal defects in conditional mutant embryos lacking the Smoothened receptor gene. Finally, a collaborative paradigm is presented that allows sharing of data across the scientific community. This work makes magnetic resonance microscopy of the mouse embryo and neonate broadly available with carefully annotated normative data and an extensive environment for collaborations.

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  • Applying parallel imaging and SMS techniques to studies in the mouse brain is challenging due to the small coil size and limited space in the probe.

    “Applying these techniques to studies in the mouse brain is challenging due to the small coil size (supplemental figure 1) and the limited space in the probe (Johnson et al. 2012; Petiet et al. 2008; Larkman and Nunes 2007).”

    From Neurite orientation dispersion and density imaging of mouse brain microstructure (2019) · cites this paper as Petiet et al. 2008

    The Discussion (page unavailable) discusses limits on imaging multiple embryos, identifying the homogeneous magnetic-field volume and gradient-coil volume as constraints. Our copy does not address whether small coil size or limited probe space makes parallel imaging or SMS challenging in mouse-brain studies; searches for those techniques, coil size, and probe space found no supporting passage. (LLM, conf=0.76)

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Authors
Petiet, A. E., Kaufman, M. H., Goddeeris, M. M., Brandenburg, J., Elmore, S. A., & Johnson, G. A.
Affiliations
  • Duke University (US)
  • University of Edinburgh (GB)
  • Departments of §Cell Biology and
  • *Center for In Vivo Microscopy and
  • National Institute of Environmental Health Sciences (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2008-08-26
Volume
105
Issue
34
Pages
12331-12336
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.0805747105
PMC
PMC2527911
OpenAlex
W2075447979
Cited by
120
References
32
Citations by year
2026: 2 · 2025: 2 · 2024: 7 · 2023: 3 · 2022: 6 · 2021: 5 · 2020: 2 · 2019: 5 · 2018: 4 · 2017: 4 · 2016: 6 · 2015: 4 · 2014: 7 · 2013: 8 · 2012: 10
FWCI
11.26
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Cancer Institute (U24CA092656)
  • National Center for Research Resources (P41RR013642, P41RR005959, U24RR021760)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD042803)
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
  • Physical Sciences › Engineering › Biomedical Engineering › Anatomy and Medical Technology
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Fetal and Pediatric Neurological Disorders
Keywords
magnetic resonance microscopy, mouse embryo, neonatal mice, developmental morphology, transgenic mouse model, mutant mouse models, cardiac septal defects, Smoothened receptor, conditional mutants, reference values, web-accessible database, data sharing
MeSH terms
Animals, Animals, Newborn, Embryo, Mammalian, Heart Septal Defects, Magnetic Resonance Imaging, Mice, Mutant Strains, Mice, Transgenic, Microscopy, Time Factors, Databases, Factual, Anatomy, Cross-Sectional, Imaging, Three-Dimensional, Embryonic Development, Mice

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