The DOI matches its Crossref record; 71 of the 71 references it cites were found in a registry or an index.
Nitrate-responsive miR393/ AFB3 regulatory module controls root system architecture in Arabidopsis thaliana
Open accessAbstract
One of the most striking examples of plant developmental plasticity to changing environmental conditions is the modulation of root system architecture (RSA) in response to nitrate supply. Despite the fundamental and applied significance of understanding this process, the molecular mechanisms behind nitrate-regulated changes in developmental programs are still largely unknown. Small RNAs (sRNAs) have emerged as master regulators of gene expression in plants and other organisms. To evaluate the role of sRNAs in the nitrate response, we sequenced sRNAs from control and nitrate-treated Arabidopsis seedlings using the 454 sequencing technology. miR393 was induced by nitrate in these experiments. miR393 targets transcripts that code for a basic helix-loop-helix (bHLH) transcription factor and for the auxin receptors TIR1, AFB1, AFB2, and AFB3. However, only AFB3 was regulated by nitrate in roots under our experimental conditions. Analysis of the expression of this miR393/ AFB3 module, revealed an incoherent feed-forward mechanism that is induced by nitrate and repressed by N metabolites generated by nitrate reduction and assimilation. To understand the functional role of this N-regulatory module for plant development, we analyzed the RSA response to nitrate in AFB3 insertional mutant plants and in miR393 overexpressors. RSA analysis in these plants revealed that both primary and lateral root growth responses to nitrate were altered. Interestingly, regulation of RSA by nitrate was specifically mediated by AFB3 , indicating that miR393/ AFB3 is a unique N-responsive module that controls root system architecture in response to external and internal N availability in Arabidopsis .
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Bartel (2004) · doi:10.1016/s0092-8674(04)00045-5 · Cited by 34,714
Genome sequencing in microfabricated high-density picolitre reactors
Margulies, M., Egholm, M., Altman, W. E., Attiya, S., Bader, J. S., Bemben, L. A., Berka, J., Braverman, M. S., Chen, Y.-J., Chen, Z., et al. (2005) · Nature · doi:10.1038/nature03959 · Cited by 7,693
Network motifs in the transcriptional regulation network of Escherichia coli
Shen-Orr, S. S., Milo, R., Mangan, S., & Alon, U. (2002) · Nature Genetics · doi:10.1038/ng881 · Cited by 2,952
MicroRNAs AND THEIR REGULATORY ROLES IN PLANTS
Jones-Rhoades, M. W., Bartel, D. P., & Bartel, B. (2006) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.57.032905.105218 · Cited by 2,686
Local, Efflux-Dependent Auxin Gradients as a Common Module for Plant Organ Formation
Benková, E., Michniewicz, M., Sauer, M., Teichmann, T., Seifertová, D., Jürgens, G., & Friml, J. (2003) · Cell · doi:10.1016/s0092-8674(03)00924-3 · Cited by 2,633
Computational Identification of Plant MicroRNAs and Their Targets, Including a Stress-Induced miRNA
Jones-Rhoades, M. W., & Bartel, D. P. (2004) · Molecular Cell · doi:10.1016/j.molcel.2004.05.027 · Cited by 2,284
Origin, Biogenesis, and Activity of Plant MicroRNAs
Voinnet, O. (2009) · Cell · doi:10.1016/j.cell.2009.01.046 · Cited by 2,212
A Plant miRNA Contributes to Antibacterial Resistance by Repressing Auxin Signaling
Navarro, L., Dunoyer, P., Jay, F., Arnold, B., Dharmasiri, N., Estelle, M., Voinnet, O., & Jones, J. D. G. (2006) · Science · doi:10.1126/science.1126088 · Cited by 1,971
Novel and Stress-Regulated MicroRNAs and Other Small RNAs from Arabidopsis[W]
Sunkar, R., & Zhu, J.-K. (2004) · The Plant Cell · doi:10.1105/tpc.104.022830 · Cited by 1,935
Structure and function of the feed-forward loop network motif
Mangan, S., & Alon, U. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2133841100 · Cited by 1,827
Cited by
Plant Nitrogen Assimilation and Use Efficiency
Xu, G., Fan, X., & Miller, A. J. (2012) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-042811-105532 · Cited by 2,114
Lateral root development in Arabidopsis: fifty shades of auxin
Lavenus, J., Goh, T., Roberts, I., Guyomarc’h, S., Lucas, M., De Smet, I., Fukaki, H., Beeckman, T., Bennett, M., & Laplaze, L. (2013) · Trends in Plant Science · doi:10.1016/j.tplants.2013.04.006 · Cited by 639
Xia, K., Wang, R., Ou, X., Fang, Z., Tian, C., Duan, J., Wang, Y., & Zhang, M. (2012) · PLoS ONE · doi:10.1371/journal.pone.0030039 · Cited by 393
Nitrate in 2020: Thirty Years from Transport to Signaling Networks
Vidal, E. A., Alvarez, J. M., Araus, V., Riveras, E., Brooks, M. D., Krouk, G., Ruffel, S., Lejay, L., Crawford, N. M., Coruzzi, G. M., et al. (2020) · The Plant Cell · doi:10.1105/tpc.19.00748 · Cited by 382
Self-citationDo plants need nitrate? The mechanisms by which nitrogen form affects plants
Andrews, M., Raven, J. A., & Lea, P. J. (2013) · Annals of Applied Biology · doi:10.1111/aab.12045 · Cited by 374
Metadata
- Authors
- Vidal, E. A., Araus, V., Lu, C., Parry, G., Green, P. J., Coruzzi, G. M., & Gutiérrez, R. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2010-02-08
- Volume
- 107
- Issue
- 9
- Pages
- 4477-4482
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2034750140
- Cited by
- 607
- References
- 71
- FWCI
- 34.20
- Type
- journal article
- Language
- en
- Open access
- green
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