Mapping amorphous calcium phosphate transformation into crystalline mineral from the cell to the bone in zebrafish fin rays
Open accessAbstract
The continuously forming fin bony rays of zebrafish represent a simple bone model system in which mineralization is temporally and spatially resolved. The mineralized collagen fibrils of the fin bones are identical in structure to those found in all known bone materials. We study the continuous mineralization process within the tissue by using synchrotron microbeam x-ray diffraction and small-angle scattering, combined with cryo-scanning electron microscopy. The former provides information on the mineral phase and the mineral particles size and shape, whereas the latter allows high-resolution imaging of native hydrated tissues. The integration of the two techniques demonstrates that new mineral is delivered and deposited as packages of amorphous calcium phosphate nanospheres, which transform into platelets of crystalline apatite within the collagen matrix.
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Cryo-EM and HPF-FS preparation have recently provided evidence of a secretory process for bone mineralization.
“Cryo-EM and HPF-FS preparation have recently provided evidence of a secretory process for bone mineralization [131, 133, 154, 155].”
From A Review of Phosphate Mineral Nucleation in Biology and Geobiology (2013) · cites this paper as [155]
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- Authors
- Mahamid, J., Aichmayer, B., Shimoni, E., Ziblat, R., Li, C., Siegel, S., Paris, O., Fratzl, P., Weiner, S., & Addadi, L.
- Affiliations
- Weizmann Institute of Science (IL)
- Max Planck Institute of Colloids and Interfaces (DE)
- Department of Structural Biology and
- Max Planck Society (DE)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2010-03-22
- Volume
- 107
- Issue
- 14
- Pages
- 6316-6321
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2851957
- OpenAlex
- W1976288179
- Cited by
- 452
- References
- 2
- Citations by year
- 2026: 9 · 2025: 19 · 2024: 20 · 2023: 27 · 2022: 17 · 2021: 31 · 2020: 42 · 2019: 28 · 2018: 32 · 2017: 30 · 2016: 45 · 2015: 37 · 2014: 24 · 2013: 36 · 2012: 26
- FWCI
- 11.76
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Max Planck Digital Library (submittedVersion)
- HZB Repository (Helmholtz-Zentrum Berlin für Materialien und Energie GmbH (HZB)) (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Calcium Carbonate Crystallization and Inhibition
- Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Alkaline Phosphatase Research Studies
- Keywords
- amorphous calcium phosphate, apatite crystallization, bone mineralization, collagen fibrils, mineral deposition, nanospheres, mineral transformation, small-angle X-ray scattering, cryo-scanning electron microscopy
- MeSH terms
- Animals, Bone and Bones, Calcification, Physiologic, Calcium Phosphates, Male, Microscopy, Electron, Scanning, Scattering, Radiation, X-Ray Diffraction, Zebrafish, Cryoelectron Microscopy
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