Nonsaturation of AMPA and NMDA receptors at hippocampal synapses
Open accessAbstract
An important issue in synaptic physiology is the extent to which postsynaptic receptors are saturated by the neurotransmitter released from a single synaptic vesicle. Although the bulk of evidence supports receptor saturation, recent studies have started to reveal that alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) and N-methyl-D-aspartate (NMDA) receptors may not be saturated by a single vesicle of glutamate. Here, we address this question through a study of putative single synapses, made by hippocampal neurons in culture, that are identified by FM1-43 staining. An analysis of the sources of variability in the amplitudes of miniature excitatory postsynaptic currents at single synapses reveals that this variability must arise presynaptically, from variations in the quantity of agonist released. Thus, glutamate receptors at hippocampal synapses are not generally saturated by quantal release.
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Postsynaptic AMPA receptors in mushroom spines are not saturated by the glutamate released from a single synaptic vesicle.
“As a corollary, our data suggest that the postsynaptic AMPA receptors in the mushroom spines are not saturated by the glutamate released from a single synaptic vesicle23, 33, because the opening probability of the AMPA receptors was estimated as 0.27, given that the largest mEPSC was ~19 pA (n = 222) in our experiments, and that such mEPSC likely occurred in the mushroom spines with largest number”
From Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons (2001) · cites this paper as [33]
The functional mapping approach was able to detect the clustering of AMPA receptors in the postsynaptic membrane.
“Thus, our functional mapping approach was able to detect the clustering of AMPA receptors in the postsynaptic membrane23, 32, 33.”
From Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons (2001) · cites this paper as [33]
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References 37 · Cited by 219
- Authors
- McAllister, A. K., & Stevens, C. F.
- Affiliations
- Howard Hughes Medical Institute (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2000-05-16
- Volume
- 97
- Issue
- 11
- Pages
- 6173-6178
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC18577
- OpenAlex
- W2105829350
- Cited by
- 219
- References
- 37
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 4 · 2023: 4 · 2022: 6 · 2021: 4 · 2020: 9 · 2019: 8 · 2018: 4 · 2017: 4 · 2016: 2 · 2015: 10 · 2014: 6 · 2013: 6 · 2012: 5
- FWCI
- 7.09
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Keywords
- AMPA receptors, NMDA receptor, receptor saturation, quantal release, miniature excitatory postsynaptic currents, hippocampal synapses, glutamate, single synapse, synaptic vesicles
- MeSH terms
- Animals, Binding, Competitive, Cells, Cultured, Dose-Response Relationship, Drug, Iontophoresis, Synaptic Transmission, Synapses, Receptors, N-Methyl-D-Aspartate, N-Methylaspartate, Dizocilpine Maleate, Pyramidal Cells, Receptors, AMPA, Patch-Clamp Techniques, Excitatory Amino Acid Agonists, Excitatory Amino Acid Antagonists, Glutamic Acid, 6-Cyano-7-nitroquinoxaline-2,3-dione, Rats
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