RETRACTED: Production and secretion of fatty acids in genetically engineered cyanobacteria
Abstract
Our purpose is to apply a fatty acid secretion strategy in photosynthetic microbial biofuel production, which will avoid the costly biomass recovery processes currently applied in algal biofuel systems. Starting with introducing acyl-acyl carrier protein thioesterases, we made five successive generations of genetic modifications into cyanobacterium Synechocystis sp. PCC 6803. The mutant strains were able to overproduce fatty acids (C10–C18) and secrete them into the medium at an efficiency of up to 133 ± 12 mg/L of culture per day at a cell density of 1.5 × 10 8 cells/mL (0.23 g of dry weight/ liter). Fatty acid secretion yields were increased by weakening the S layer and peptidoglycan layers. Although the fatty acid secreting strains had a long lag phase with many cells having damaged cell membranes when grown at low cell densities, these strains grew more rapidly in stationary phase and exhibited less cell damage than wild-type in a stationary culture. Our results suggest that fatty acid secreting cyanobacteria are a promising technology for renewable biofuel production.
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Cyanobacteria have been engineered to secrete as much as 133 mg/l/day of lipids.
“Cyanobacteria have been engineered to secrete as much as 133 mg/l/day [155]”
From Biofuels from algae: Challenges and potential (2010) · cites this paper as [155]
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References 36 · Cited by 33
- Authors
- Liu, X., Brune, D., Vermaas, W., & Curtiss, R.
- Affiliations
- Arizona State University (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2026-01-01
- Issue
- 0
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2150882201
- Cited by
- 33
- References
- 36
- Citations by year
- 2023: 1 · 2022: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 6 · 2012: 6
- FWCI
- 3.59
- Type
- journal article
- Language
- en
- Status
- Retracted
- Update notices
- retraction (2010-07-02)
- Open access
- closed
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- fatty acid secretion, cyanobacteria, Synechocystis sp. PCC 6803, algal biofuels, genetic engineering, S-layer, peptidoglycan, cell membrane damage, lag phase, biomass recovery
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