The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant
Open accessAbstract
Juvenile hormone (JH) is a sesquiterpenoid of vital importance for insect development, yet the molecular basis of JH signaling remains obscure, mainly because a bona fide JH receptor has not been identified. Mounting evidence points to the basic helix–loop–helix (bHLH)/Per-Arnt-Sim (PAS) domain protein Methoprene-tolerant (Met) as the best JH receptor candidate. However, details of how Met transduces the hormonal signal are missing. Here, we demonstrate that Met specifically binds JH III and its biologically active mimics, methoprene and pyriproxyfen, through its C-terminal PAS domain. Substitution of individual amino acids, predicted to form a ligand-binding pocket, with residues possessing bulkier side chains reduces JH III binding likely because of steric hindrance. Although a mutation that abolishes JH III binding does not affect a Met–Met complex that forms in the absence of methoprene, it prevents both the ligand-dependent dissociation of the Met–Met dimer and the ligand-dependent interaction of Met with its partner bHLH-PAS protein Taiman. These results show that Met can sense the JH signal through direct, specific binding, thus establishing a unique class of intracellular hormone receptors.
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Cites
Trott, O., & Olson, A. J. (2010) · Journal of Computational Chemistry · doi:10.1002/jcc.21334 · Cited by 38,412
Dundas, J., Ouyang, Z., Tseng, J., Binkowski, A., Turpaz, Y., & Liang, J. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl282 · Cited by 1,725
Artificial ligand binding within the HIF2α PAS-B domain of the HIF2 transcription factor
Scheuermann, T. H., Tomchick, D. R., Machius, M., Guo, Y., Bruick, R. K., & Gardner, K. H. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0808092106 · Cited by 301
Konopova, B., Smykal, V., & Jindra, M. (2011) · PLoS ONE · doi:10.1371/journal.pone.0028728 · Cited by 250
Self-citationPandini, A., Denison, M. S., Song, Y., Soshilov, A. A., & Bonati, L. (2007) · Biochemistry · doi:10.1021/bi061460t · Cited by 160 · cited 2×
“Finally, substitution of A375 in the center of the AhR ligand-binding pocket with bulkier residues prevents TCDD binding because of steric hindrance (23).”
BackgroundPandini, A., Soshilov, A. A., Song, Y., Zhao, J., Bonati, L., & Denison, M. S. (2009) · Biochemistry · doi:10.1021/bi900259z · Cited by 124
Cited by
The Juvenile Hormone Signaling Pathway in Insect Development
Jindra, M., Palli, S. R., & Riddiford, L. M. (2013) · Annual Review of Entomology · doi:10.1146/annurev-ento-120811-153700 · Cited by 874
Self-citationMolecular basis of juvenile hormone signaling
Jindra, M., Bellés, X., & Shinoda, T. (2015) · Current Opinion in Insect Science · doi:10.1016/j.cois.2015.08.004 · Cited by 281
Self-citationImportance of juvenile hormone signaling arises with competence of insect larvae to metamorphose
Smykal, V., Daimon, T., Kayukawa, T., Takaki, K., Shinoda, T., & Jindra, M. (2014) · Developmental Biology · doi:10.1016/j.ydbio.2014.03.006 · Cited by 135
Self-citationHuang, J.-H., Lozano, J., & Belles, X. (2013) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2012.09.025 · Cited by 101
Kayukawa, T., Murata, M., Kobayashi, I., Muramatsu, D., Okada, C., Uchino, K., Sezutsu, H., Kiuchi, M., Tamura, T., Hiruma, K., et al. (2014) · Developmental Biology · doi:10.1016/j.ydbio.2014.01.022 · Cited by 97
Metadata
- Authors
- Charles, J.-P., Iwema, T., Epa, V. C., Takaki, K., Rynes, J., & Jindra, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2011-12-13
- Volume
- 108
- Issue
- 52
- Pages
- 21128-21133
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2065469900
- Cited by
- 382
- References
- 6
- FWCI
- 7.86
- Type
- journal article
- Language
- en
- Open access
- green
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- Charles, J.-P., Iwema, T., Epa, V. C., Takaki, K., Rynes, J., & Jindra, M. (2011). Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1116123109
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