The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Biochemical analysis of a multifunctional cytochrome P450 (CYP51) enzyme required for synthesis of antimicrobial triterpenes in plants
Open accessAbstract
Significance: We carried out functional analysis of the oat enzyme AsCYP51H10, which is a divergent member of the CYP51 cytochrome P450 family and showed that this enzyme is able to catalyze both hydroxylation and epoxidation of the simple triterpene β-amyrin to give 12,13β-epoxy-3β,16β-dihydroxy-oleanane (12,13β-epoxy-16β-hydroxy-β-amyrin). In contrast, the canonical CYP51 enzymes are highly conserved and catalyze only sterol demethylation. We further show that the C12,13 epoxy group is critical for antifungal activity, a discovery that has important implications for triterpene metabolic engineering for food, health, and industrial biotechnology applications.
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Trott, O., & Olson, A. J. (2010) · Journal of Computational Chemistry · doi:10.1002/jcc.21334 · Cited by 38,412
Cited by
Triterpene Biosynthesis in Plants
Thimmappa, R., Geisler, K., Louveau, T., O'Maille, P., & Osbourn, A. (2014) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050312-120229 · Cited by 803
Self-citationThe cytochrome P450 superfamily: Key players in plant development and defense
XU, J., WANG, X.-Y., & GUO, W.-Z. (2015) · Journal of Integrative Agriculture · doi:10.1016/s2095-3119(14)60980-1 · Cited by 355
Gene clustering in plant specialized metabolism
Nützmann, H.-W., & Osbourn, A. (2014) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2013.10.009 · Cited by 255
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Metadata
- Authors
- Geisler, K., Hughes, R. K., Sainsbury, F., Lomonossoff, G. P., Rejzek, M., Fairhurst, S., Olsen, C.-E., Motawia, M. S., Melton, R. E., Hemmings, A. M., Bak, S., & Osbourn, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2013-08-12
- Volume
- 110
- Issue
- 35
- Pages
- E3360-7
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2072553353
- Cited by
- 183
- References
- 1
- FWCI
- 4.98
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Geisler, K., Hughes, R. K., Sainsbury, F., Lomonossoff, G. P., Rejzek, M., Fairhurst, S., Olsen, C.-E., Motawia, M. S., Melton, R. E., Hemmings, A. M., Bak, S., & Osbourn, A. (2013). Biochemical analysis of a multifunctional cytochrome P450 (CYP51) enzyme required for synthesis of antimicrobial triterpenes in plants. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1309157110
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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