The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Structural and functional characterization of a conserved pair of bacterial cellulose-oxidizing lytic polysaccharide monooxygenases
Open accessAbstract
Significance: The discovery of lytic polysaccharide monooxygenases (LPMOs) has profoundly changed our understanding of the enzymatic conversion of recalcitrant polysaccharides, such as cellulose. Although in-depth studies of fungal cellulolytic LPMOs have been reported, the structures and functions of their bacterial counterparts with no detectable sequence similarity remain largely elusive. We present the structures of a conserved pair of bacterial cellulose-active LPMOs supplemented with extensive functional characterization. The structural data allow a thorough comparative assessment of fungal and bacterial LPMOs, providing insight into the structural basis of substrate specificity and the oxidative mechanism (C1/C4 oxidation). Importantly, we show that this LPMO pair acts synergistically when degrading cellulose, a finding that may help explain the occurrence of multiple LPMOs in a single microbe.
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Adams, P. D., Afonine, P. V., Bunkóczi, G., Chen, V. B., Davis, I. W., Echols, N., Headd, J. J., Hung, L.-W., Kapral, G. J., Grosse-Kunstleve, R. W., et al. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909052925 · Cited by 23,716
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Primary metabolism and its control in streptomycetes: A most unusual group of bacteria
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Cited by
Payne, C. M., Knott, B. C., Mayes, H. B., Hansson, H., Himmel, M. E., Sandgren, M., Ståhlberg, J., & Beckham, G. T. (2015) · Chemical Reviews · doi:10.1021/cr500351c · Cited by 855
Oxidative cleavage of polysaccharides by monocopper enzymes depends on H2O2
Bissaro, B., Røhr, Å. K., Müller, G., Chylenski, P., Skaugen, M., Forsberg, Z., Horn, S. J., Vaaje-Kolstad, G., & Eijsink, V. G. H. (2017) · Nature Chemical Biology · doi:10.1038/nchembio.2470 · Cited by 594
Self-citationLytic Polysaccharide Monooxygenases in Biomass Conversion
Hemsworth, G. R., Johnston, E. M., Davies, G. J., & Walton, P. H. (2015) · Trends in Biotechnology · doi:10.1016/j.tibtech.2015.09.006 · Cited by 314
Eibinger, M., Ganner, T., Bubner, P., Rošker, S., Kracher, D., Haltrich, D., Ludwig, R., Plank, H., & Nidetzky, B. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.m114.602227 · Cited by 286
Sabbadin, F., Hemsworth, G. R., Ciano, L., Henrissat, B., Dupree, P., Tryfona, T., Marques, R. D. S., Sweeney, S. T., Besser, K., Elias, L., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-03142-x · Cited by 282
Metadata
- Authors
- Forsberg, Z., Mackenzie, A. K., Sørlie, M., Røhr, Å. K., Helland, R., Arvai, A. S., Vaaje-Kolstad, G., & Eijsink, V. G. H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2014-05-27
- Volume
- 111
- Issue
- 23
- Pages
- 8446-8451
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2160604830
- Cited by
- 289
- References
- 4
- FWCI
- 14.57
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Forsberg, Z., Mackenzie, A. K., Sørlie, M., Røhr, Å. K., Helland, R., Arvai, A. S., Vaaje-Kolstad, G., & Eijsink, V. G. H. (2014). Structural and functional characterization of a conserved pair of bacterial cellulose-oxidizing lytic polysaccharide monooxygenases. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1402771111
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