The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
DNA barcoding and metabarcoding of standardized samples reveal patterns of marine benthic diversity
Open accessAbstract
Significance: High-throughput DNA sequencing methods are revolutionizing our ability to census communities, but most analyses have focused on microbes. Using an environmental DNA sequencing approach based on cytochrome c oxidase subunit 1 primers, we document the enormous diversity and fine-scale geographic structuring of the cryptic animals living on oyster reefs, many of which are rare and very small. Sequence data reflected both the presence and relative abundance of organisms, but only 10.9% of the sequences could be matched to reference barcodes in public databases. These results highlight the enormous numbers of marine animal species that remain genetically unanchored to conventional taxonomy and the importance of standardized, genetically based biodiversity surveys to monitor global change.
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Basic local alignment search tool
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QIIME allows analysis of high-throughput community sequencing data
Caporaso, J. G., Kuczynski, J., Stombaugh, J., Bittinger, K., Bushman, F. D., Costello, E. K., Fierer, N., Peña, A. G., Goodrich, J. K., Gordon, J. I., et al. (2010) · Nature Methods · doi:10.1038/nmeth.f.303 · Cited by 34,887
A new method for non-parametric multivariate analysis of variance
Anderson, M. J. (2001) · Austral Ecology · doi:10.1111/j.1442-9993.2001.01070.pp.x · Cited by 13,828
How Many Species Are There on Earth and in the Ocean?
Mora, C., Tittensor, D. P., Adl, S., Simpson, A. G. B., & Worm, B. (2011) · PLoS Biology · doi:10.1371/journal.pbio.1001127 · Cited by 3,045
Global patterns and predictors of marine biodiversity across taxa
Tittensor, D. P., Mora, C., Jetz, W., Lotze, H. K., Ricard, D., Berghe, E. V., & Worm, B. (2010) · Nature · doi:10.1038/nature09329 · Cited by 1,524
Cited by
Scrutinizing key steps for reliable metabarcoding of environmental samples
Alberdi, A., Aizpurua, O., Gilbert, M. T. P., & Bohmann, K. (2018) · Methods in Ecology and Evolution · doi:10.1111/2041-210x.12849 · Cited by 688
Elbrecht, V., Vamos, E. E., Meissner, K., Aroviita, J., & Leese, F. (2017) · Methods in Ecology and Evolution · doi:10.1111/2041-210x.12789 · Cited by 385
Levy Karin, E., Mirdita, M., & Söding, J. (2020) · Microbiome · doi:10.1186/s40168-020-00808-x · Cited by 299
“Since the advent of metabarcoding using 18S rRNA genes, the known evolutionary diversity 53 of unicellular eukaryotes has increased by orders of magnitude [13], and novel phyla and 54 supra-kingdoms are still being discovered [14,15].”
BackgroundWangensteen, O. S., Palacín, C., Guardiola, M., & Turon, X. (2018) · PeerJ · doi:10.7717/peerj.4705 · Cited by 312
High Throughput Sequencing for Detection of Foodborne Pathogens
Sekse, C., Holst-Jensen, A., Dobrindt, U., Johannessen, G. S., Li, W., Spilsberg, B., & Shi, J. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.02029 · Cited by 98
Metadata
- Authors
- Leray, M., & Knowlton, N.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2015-02-02
- Volume
- 112
- Issue
- 7
- Pages
- 2076-2081
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2071413362
- Cited by
- 597
- References
- 5
- FWCI
- 51.00
- Type
- journal article
- Language
- en
- Open access
- green
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- Leray, M., & Knowlton, N. (2015). DNA barcoding and metabarcoding of standardized samples reveal patterns of marine benthic diversity. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1424997112
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.