Mouse embryonic fibroblasts exhibit extensive developmental and phenotypic diversity
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Significance: Embryonic fibroblasts have long been considered a relatively homogeneous cell type, and many studies have been conducted on fibroblasts of varying provenance. The results of the present study indicate that embryonic fibroblasts are highly heterogeneous and exhibit anatomic and developmental variation that has both practical consequences for the interpretation of experimental results and theoretical implications for the nature and organization of metazoan organisms. Studies using embryonic fibroblasts to explore the consequences of altered alleles should take into account the possibility that fibroblast composition may be altered by allele expression.
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Mouse embryonic skin mesenchymal and epithelial cells are composed of heterogeneous cell populations.
“Because both mesenchymal and epithelial cells from mouse embryonic skin are composed of heterogeneous cell populations (52, 53)”
From Reprogramming of three-dimensional microenvironments for in vitro hair follicle induction (2022) · cites this paper as [52]
The Significance section reports that mouse embryonic fibroblasts are highly heterogeneous, supporting the mesenchymal-cell portion at a broader level. The cited passages do not establish heterogeneity specifically in embryonic skin or in its epithelial cells. (LLM, conf=0.87)
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Reprogramming of three-dimensional microenvironments for in vitro hair follicle induction
Kageyama, T., Shimizu, A., Anakama, R., Nakajima, R., Suzuki, K., Okubo, Y., & Fukuda, J. (2022) · Science Advances · doi:10.1126/sciadv.add4603 · Cited by 73 · see citing works
- Authors
- Singhal, P. K., Sassi, S., Lan, L., Au, P., Halvorsen, S. C., Fukumura, D., Jain, R. K., & Seed, B.
- Affiliations
- Harvard University (US)
- Massachusetts General Hospital (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2015-12-22
- Volume
- 113
- Issue
- 1
- Pages
- 122-127
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC4711836
- OpenAlex
- W2228436275
- Cited by
- 66
- References
- 1
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 8 · 2023: 5 · 2022: 5 · 2021: 5 · 2020: 13 · 2019: 7 · 2018: 8 · 2017: 4 · 2016: 3
- FWCI
- 2.17
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion, other-oa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
- Funders
- HHS | NIH | National Cancer Institute (P01CA080124, R35CA197743)
- HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (R01DK103879)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Keywords
- embryonic fibroblasts, fibroblast heterogeneity, fibroblast subtypes, multipotency, surface phenotype, fibroblast-like cells, GFP reporter mice, cell sorting, cell culture propagation
- MeSH terms
- Animals, Cell Line, Cell Separation, Embryo, Mammalian, Fibroblasts, Flow Cytometry, Mice, Transgenic, Phenotype, Pericytes, Vascular Endothelial Growth Factor A, Green Fluorescent Proteins, Mice, Single-Cell Analysis, Transcriptome
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