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In vivo immunological function of mast cells derived from embryonic stem cells: An approach for the rapid analysis of even embryonic lethal mutations in adult mice in vivo
Abstract
An important goal of tissue engineering is to achieve reconstitution of specific functionally active cell types by transplantation of differentiated cell populations derived from normal or genetically altered embryonic stem cells in vitro . We find that mast cells derived in vitro from wild-type or genetically manipulated embryonic stem cells can survive and orchestrate immunologically specific IgE-dependent reactions after transplantation into mast cell-deficient Kit W / Kit W-v mice. These findings define a unique approach for analyzing the effects of mutations of any genes that are expressed in mast cells, including embryonic lethal mutations, in vitro or in vivo .
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“Moreover, Tsai et al. showed that one also can generate such mast cells from embryonic stem cells, permitting the analysis in vivo of mast cells which lack products which, if absent in the germ line, would result in embryonic or perinatal lethality [64].”
From Mast cells and IgE in defense against lethality of venoms: Possible “benefit” of allergy (2020) · cites this paper as [64]
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Moon, K. R., van Dijk, D., Wang, Z., Gigante, S., Burkhardt, D. B., Chen, W. S., Yim, K., Elzen, A. V. D., Hirn, M. J., Coifman, R. R., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0336-3 · Cited by 1,065
Yu, C., Cantor, A. B., Yang, H., Browne, C., Wells, R. A., Fujiwara, Y., & Orkin, S. H. (2002) · The Journal of Experimental Medicine · doi:10.1084/jem.20020656 · Cited by 600
“To assess mast cell production in dblGATA mice, we cultured wt or hemizygous dblGATA bone marrow cells in medium containing stem cell factor and IL-3 to obtain mast cells (17).”
MethodMast cells and IgE in defense against venoms: Possible “good side” of allergy?
Galli, S. J., Starkl, P., Marichal, T., & Tsai, M. (2016) · Allergology International · doi:10.1016/j.alit.2015.09.002 · Cited by 73
Self-citationMast cells and IgE in defense against lethality of venoms: Possible “benefit” of allergy
Galli, S. J., Metz, M., Starkl, P., Marichal, T., & Tsai, M. (2020) · Allergo Journal International · doi:10.1007/s40629-020-00118-6 · Cited by 57
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Metadata
- Authors
- Tsai, M., Wedemeyer, J., Ganiatsas, S., Tam, S.-Y., Zon, L. I., & Galli, S. J.
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2000-07-25
- Volume
- 97
- Issue
- 16
- Pages
- 9186-9190
- ISSN
- 0027-8424
- 1091-6490
- Cited by
- 70
- References
- 1
- Language
- en
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