Transcriptomes of major renal collecting duct cell types in mouse identified by single-cell RNA-seq
Open accessPublished version of a preprint
Abstract
Significance: A long-term goal in mammalian biology is to identify the genes expressed in every cell type of the body. In the kidney, the expressed genes (i.e., transcriptome) of all epithelial cell types have already been identified with the exception of the cells that make up the renal collecting duct, which is responsible for regulation of blood pressure and body fluid composition. Here, single-cell RNA-sequencing was used in mouse to identify transcriptomes for the major collecting duct cell types: type A intercalated cells, type B intercalated cells, and principal cells. The information was used to create a publicly accessible online resource. The data allowed identification of genes that are selectively expressed in each cell type, which is informative for cell-level understanding of physiology and pathophysiology.
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References 45 · Cited by 267
- Authors
- Chen, L., Lee, J. W., Chou, C.-L., Nair, A. V., Battistone, M. A., Păunescu, T. G., Merkulova, M., Breton, S., Verlander, J. W., Wall, S. M., Brown, D., Burg, M. B., & Knepper, M. A.
- Affiliations
- National Institutes of Health (US)
- National Cancer Center (KR)
- Harvard University (US)
- Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114;
- University of Florida Health (US)
- Emory University (US)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2017-10-31
- Volume
- 114
- Issue
- 46
- Pages
- E9989-E9998
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC5699061
- OpenAlex
- W2750899741
- Cited by
- 267
- References
- 45
- Citations by year
- 2026: 17 · 2025: 24 · 2024: 42 · 2023: 24 · 2022: 24 · 2021: 33 · 2020: 47 · 2019: 27 · 2018: 25 · 2017: 3 · 2015: 1
- FWCI
- 11.34
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- HHS | NIH | National Heart, Lung, and Blood Institute (ZIA-HL001285, ZIA-HL006129)
- HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (R01-DK107798, R01-DK104125, 4R37 DK042956-24)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Renin-Angiotensin System Studies
- Keywords
- renal collecting duct, intercalated cells, principal cells, cell type transcriptomes, renal epithelial cells, single-cell RNA sequencing, mice, cell type-specific gene expression
- MeSH terms
- Jagged-1 Protein, Sulfate Transporters, Animals, Anion Exchange Protein 1, Erythrocyte, Base Sequence, Epithelial Cells, Kidney, Kidney Tubules, Collecting, Male, Membrane Proteins, Mice, Inbred C57BL, RNA, Transcription Factors, Signal Transduction, Biomarkers, Gene Expression, Sequence Analysis, RNA, Gene Expression Profiling, Anion Transport Proteins, Mice, Aquaporin 2, Receptor, Notch2, Transcriptome
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