The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
cAMP acts as a second messenger in pollen tube growth and reorientation
Open accessAbstract
Pollen tube growth and reorientation is a prerequisite for fertilization and seed formation. Here we report imaging of cAMP distribution in living pollen tubes microinjected with the protein kinase A-derived fluorosensor. Growing tubes revealed a uniform distribution of cAMP with a resting concentration of ≈100–150 nM. Modulators of adenylyl cyclase (AC), forskolin, and dideoxyadenosine could alter these values. Transient elevations in the apical region could be correlated with changes in the tube-growth axis, suggesting a role for cAMP in polarized growth. Changes in cAMP arise through the activity of a putative AC identified in pollen. This signaling protein shows homology to functional motifs in fungal AC. Expression of the cDNA in Escherichia coli resulted in cAMP increase and complemented a catabolic defect in the fermentation of carbohydrates caused by the absence of cAMP in a cya A mutant. Antisense assays performed with oligodeoxynucleotide probes directed against conserved motifs perturbed tip growth, suggesting that modulation of cAMP concentration is vital for tip growth.
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Cites
Soluble Adenylyl Cyclase as an Evolutionarily Conserved Bicarbonate Sensor
Chen, Y., Cann, M. J., Litvin, T. N., Iourgenko, V., Sinclair, M. L., Levin, L. R., & Buck, J. (2000) · Science · doi:10.1126/science.289.5479.625 · Cited by 846
Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals
Buck, J., Sinclair, M. L., Schapal, L., Cann, M. J., & Levin, L. R. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.1.79 · Cited by 527
Cited by
Nitric oxide signalling in plants
Neill, S. J., Desikan, R., & Hancock, J. T. (2003) · New Phytologist · doi:10.1046/j.1469-8137.2003.00804.x · Cited by 874
Becker, J. D., Boavida, L. C., Carneiro, J., Haury, M., & Feijó, J. A. (2003) · Plant Physiology · doi:10.1104/pp.103.028241 · Cited by 371
Nitric oxide is involved in growth regulation and re-orientation of pollen tubes
Prado, A. M., Porterfield, D. M., & Feijó, J. A. (2004) · Development · doi:10.1242/dev.01153 · Cited by 299
Cyclic nucleotides: The current dilemma
Trewavas, A. (2002) · Current Opinion in Plant Biology · doi:10.1016/s1369-5266(02)00281-9 · Cited by 27
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Metadata
- Authors
- Moutinho, A., Hussey, P. J., Trewavas, A. J., & Malhó, R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2001-08-21
- Volume
- 98
- Issue
- 18
- Pages
- 10481-10486
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1972649738
- Cited by
- 244
- References
- 2
- FWCI
- 4.67
- Type
- journal article
- Language
- en
- Open access
- green
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- Moutinho, A., Hussey, P. J., Trewavas, A. J., & Malhó, R. (2001). cAMP acts as a second messenger in pollen tube growth and reorientation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.171104598
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