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Cited by 53Lipid Membrane Structure and BehaviorAdvanced Fluorescence Microscopy TechniquesSpectroscopy and Quantum Chemical Studies

Label-free and charge-sensitive dynamic imaging of lipid membrane hydration on millisecond time scales

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Tarun, Orly B., Hannesschläger, Christof, Pohl, Peter, Roke, Sylvie·2018·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.1719347115PMC PMC5910843
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Abstract

Significance: Lipid bilayer membranes are responsible for compartmentalization, signaling, transport, and flow of charge in living cells. Membranes self-assemble in aqueous solutions. Without a hydrating environment, membranes cannot exist. It is therefore surprising to note that the hydrating water is neglected in most membrane-related studies. We imaged membrane-bound oriented water by means of label-free second harmonic microscopy. We tracked, on millisecond time scales, membrane domain diffusion of condensed charged lipid domains, domain structure, and the spatial distribution of charge. Real-time electrostatic membrane potential maps were constructed using nonlinear optical theory. The spatiotemporal fluctuation in the membrane potential is surprisingly large and reveals the importance of charge fluctuations on membranes.

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  • GUV fusion occurs in the presence of transmembrane potentials but not in their absence.

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Authors
Tarun, O. B., Hannesschläger, C., Pohl, P., & Roke, S.
Affiliations
  • École Polytechnique Fédérale de Lausanne (CH)
  • Institute of Biophysics, Johannes Kepler University Linz, A-4020 Linz, Austria
  • Johannes Kepler University of Linz (AT)
Corresponding author
Roke, Sylvie
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2018-04-02
Volume
115
Issue
16
Pages
4081-4086
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.1719347115
PMC
PMC5910843
OpenAlex
W2796090647
Cited by
53
References
0
Citations by year
2026: 4 · 2025: 6 · 2024: 5 · 2023: 5 · 2022: 6 · 2021: 12 · 2020: 5 · 2019: 7 · 2018: 3
FWCI
2.36
Type
journal article
Language
en
Open access
Licence
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion, cc-by-nc-nd)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
Funders
  • Swiss National Science Foundation (200021-140472)
  • Austrian Science Fund (P23679)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
Keywords
second harmonic generation microscopy, label-free imaging, charge-dipole interactions, water orientation, membrane potential, electrostatic potential mapping, ionic strength effects, pH effect, membrane dynamics, nonlinear optics, lipid bilayers
MeSH terms
Diffusion, Lipid Bilayers, Membrane Lipids, Membrane Potentials, Time Factors, Water, Microscopy, Confocal, Hydrophobic and Hydrophilic Interactions

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