Material Microenvironmental Properties Couple to Induce Distinct Transcriptional Programs in Mammalian Stem Cells
Open accessAbstract
Significance: Cells have been shown to respond to a host of physical properties of the environments that surround them. However, given that these properties vary considerably across tissues, how these individual properties interact to form unique regulatory environments for cells is largely unknown. This work analyzes the transcriptional responses of cells to unique combinations of microenvironmental material properties to gain broad insights into the coupling among different properties, the magnitude of the transcriptional effects, and the role of cell type. We find significant coupling among these properties, large variation in the magnitude of the transcriptional changes, and qualitative differences in the responses based on cell type, demonstrating the significant context dependence of microenvironmental material sensing.
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The physical structure of cell microenvironments can alter cell morphology and function.
“as well as the physical structure of cell microenvironments, which can alter cell morphology and function (Darnell et al., 2018).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Darnell et al., 2018
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Role of YAP/TAZ in mechanotransduction
Dupont, S., Morsut, L., Aragona, M., Enzo, E., Giulitti, S., Cordenonsi, M., Zanconato, F., Le Digabel, J., Forcato, M., Bicciato, S., et al. (2011) · Nature · doi:10.1038/nature10137 · Cited by 5,979 · see citing works
Fibrous nonlinear elasticity enables positive mechanical feedback between cells and ECMs
Hall, M. S., Alisafaei, F., Ban, E., Feng, X., Hui, C.-Y., Shenoy, V. B., & Wu, M. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1613058113 · Cited by 360 · see citing works
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Effects of extracellular matrix viscoelasticity on cellular behaviour
Chaudhuri, O., Cooper-White, J., Janmey, P. A., Mooney, D. J., & Shenoy, V. B. (2020) · Nature · doi:10.1038/s41586-020-2612-2 · Cited by 2,224 · see citing works
Self-citationToward Best Practices for Controlling Mammalian Cell Culture Environments
Klein, S. G., Steckbauer, A., Alsolami, S. M., Arossa, S., Parry, A. J., Li, M., & Duarte, C. M. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.788808 · Cited by 23 · see citing works
- Authors
- Darnell, M., O’Neil, A., Mao, A., Gu, L., Rubin, L. L., & Mooney, D. J.
- Affiliations
- Harvard University (US)
- Corresponding author
- Mooney, David J.
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2018-08-17
- Volume
- 115
- Issue
- 36
- Pages
- E8368-E8377
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC6130338
- OpenAlex
- W2887937567
- Cited by
- 120
- References
- 2
- Citations by year
- 2026: 4 · 2025: 12 · 2024: 13 · 2023: 13 · 2022: 17 · 2021: 27 · 2020: 23 · 2019: 10 · 2018: 1
- FWCI
- 8.45
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- HHS | NIH | National Institute of Dental and Craniofacial Research (R01 DE013033)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- extracellular matrix stiffness, stress relaxation, mechanotransduction, transcriptional programs, mesenchymal stem cells, hydrogels, cytokine production, hematopoietic stem cell differentiation, 3D cell culture
- MeSH terms
- Alginates, Animals, Cell Differentiation, Hematopoietic Stem Cells, Hexuronic Acids, Transcription, Genetic, Cell Culture Techniques, Hydrogels, Glucuronic Acid, Mice, Stem Cell Niche, Mesenchymal Stem Cells
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