The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
The tobacco salicylic acid-binding protein 3 (SABP3) is the chloroplast carbonic anhydrase, which exhibits antioxidant activity and plays a role in the hypersensitive defense response
Open accessAbstract
In plants, salicylic acid (SA) plays an important role in signaling both local and systemic defense responses. Previous efforts to identify SA effector proteins in tobacco have led to the isolation of two soluble cytoplasmic SA-binding proteins (SABPs): catalase, SABP, and an ≈25-kDa protein, SABP2. Here we describe the identification of an SA-binding protein, SABP3, in the stroma of tobacco chloroplasts. SABP3 bound SA with an apparent dissociation constant ( K d ) of 3.7 μM and exhibited much greater affinity for biologically active than inactive analogs. Purification and partial sequencing of SABP3 indicated that it is the chloroplast carbonic anhydrase (CA). Confirming this finding, recombinant tobacco chloroplast CA exhibited both CA enzymatic and SA-binding activities. Expression of this protein in yeast also demonstrated that CA/SABP3 has antioxidant activity. A second gene encoding CA was also cloned, and its encoded protein was shown to behave similarly to that purified as SABP3. Finally, silencing of CA gene expression in leaves suppressed the Pto : avrPto -mediated hypersensitive response in disease resistance. These results demonstrate that SA may act through multiple effector proteins in plants and shed further light on the function of CA in chloroplasts.
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Cites
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Cited by
Salicylic acid, a multifaceted hormone to combat disease
Vlot, A. C., Dempsey, D. A., & Klessig, D. F. (2009) · Annual Review of Phytopathology · doi:10.1146/annurev.phyto.050908.135202 · Cited by 2,568
Self-citationSalicylic acid beyond defence: its role in plant growth and development
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Methyl Salicylate Is a Critical Mobile Signal for Plant Systemic Acquired Resistance
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Self-citationNPR3 and NPR4 are receptors for the immune signal salicylic acid in plants
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Metadata
- Authors
- Slaymaker, D. H., Navarre, D. A., Clark, D., del Pozo, O., Martin, G. B., & Klessig, D. F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2002-08-15
- Volume
- 99
- Issue
- 18
- Pages
- 11640-11645
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1984595651
- Cited by
- 393
- References
- 40
- FWCI
- 14.72
- Type
- journal article
- Language
- en
- Open access
- green
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- Slaymaker, D. H., Navarre, D. A., Clark, D., del Pozo, O., Martin, G. B., & Klessig, D. F. (2002). The tobacco salicylic acid-binding protein 3 (SABP3) is the chloroplast carbonic anhydrase, which exhibits antioxidant activity and plays a role in the hypersensitive defense response. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.182427699
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