The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Identification of endoglin as a functional marker that defines long-term repopulating hematopoietic stem cells
Open accessAbstract
We describe a strategy to obtain highly enriched long-term repopulating (LTR) hematopoietic stem cells (HSCs) from bone marrow side-population (SP) cells by using a transgenic reporter gene driven by a stem cell enhancer. To analyze the gene-expression profile of the rare HSC population, we developed an amplification protocol termed “constant-ratio PCR,” in which sample and control cDNAs are amplified in the same PCR. This protocol allowed us to identify genes differentially expressed in the enriched LTR-HSC population by oligonucleotide microarray analysis using as little as 1 ng of total RNA. Endoglin, an ancillary transforming growth factor β receptor, was differentially expressed by the enriched HSCs. Importantly, endoglin-positive cells, which account for 20% of total SP cells, contain all the LTR-HSC activity within bone marrow SP. Our results demonstrate that endoglin, which plays important roles in angiogenesis and hematopoiesis, is a functional marker that defines LTR HSCs. Our overall strategy may be applicable for the identification of markers for other tissue-specific stem cells.
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Zhou, S., Schuetz, J. D., Bunting, K. D., Colapietro, A.-M., Sampath, J., Morris, J. J., Lagutina, I., Grosveld, G. C., Osawa, M., Nakauchi, H., et al. (2001) · Nature Medicine · doi:10.1038/nm0901-1028 · Cited by 2,280
Okada, S., Wang, Z.-Q., Grigoriadis, A. E., Wagner, E. F., & von Rüden, T. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.1.382-390.1994 · Cited by 62
Cited by
Pronk, C. J. H., Rossi, D. J., Månsson, R., Attema, J. L., Norddahl, G. L., Chan, C. K. F., Sigvardsson, M., Weissman, I. L., & Bryder, D. (2007) · Cell Stem Cell · doi:10.1016/j.stem.2007.07.005 · Cited by 671
Zhao, M., Perry, J. M., Marshall, H., Venkatraman, A., Qian, P., He, X. C., Ahamed, J., & Li, L. (2014) · Nature Medicine · doi:10.1038/nm.3706 · Cited by 607
Detection of functional haematopoietic stem cell niche using real-time imaging
Xie, Y., Yin, T., Wiegraebe, W., He, X. C., Miller, D., Stark, D., Perko, K., Alexander, R., Schwartz, J., Grindley, J. C., et al. (2009) · Nature · doi:10.1038/nature07639 · Cited by 555
Awakening dormant haematopoietic stem cells
Trumpp, A., Essers, M., & Wilson, A. (2010) · Nature Reviews Immunology · doi:10.1038/nri2726 · Cited by 425
Identification and isolation of hematopoietic stem cells
Wognum, A. W., Eaves, A. C., & Thomas, T. E. (2003) · Archives of Medical Research · doi:10.1016/j.arcmed.2003.09.008 · Cited by 276
Metadata
- Authors
- Chen, C.-Z., Li, M., de Graaf, D., Monti, S., Göttgens, B., Sanchez, M.-J., Lander, E. S., Golub, T. R., Green, A. R., & Lodish, H. F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2002-11-15
- Volume
- 99
- Issue
- 24
- Pages
- 15468-15473
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1673118505
- Cited by
- 167
- References
- 2
- FWCI
- 5.06
- Type
- journal article
- Language
- en
- Open access
- green
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- Chen, C.-Z., Li, M., de Graaf, D., Monti, S., Göttgens, B., Sanchez, M.-J., Lander, E. S., Golub, T. R., Green, A. R., & Lodish, H. F. (2002). Identification of endoglin as a functional marker that defines long-term repopulating hematopoietic stem cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.202614899
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