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Cited by 16Bacterial Genetics and BiotechnologyVibrio bacteria research studiesBacterial biofilms and quorum sensing

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Eukaryotic catecholamine hormones influence the chemotactic control of Vibrio campbellii by binding to the coupling protein CheW

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Weigert Muñoz, Angela, Hoyer, Elisabeth, Schumacher, Kilian, Grognot, Marianne, Taute, Katja M., Hacker, Stephan M., Sieber, Stephan A., Jung, Kirsten·2022·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.2118227119PMC PMC8915975

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Abstract

Significance: Host-emitted stress hormones significantly influence the growth and behavior of various bacterial species; however, their cellular targets have so far remained elusive. Here, we used customized probes and quantitative proteomics to identify the target of epinephrine and the α-adrenoceptor agonist phenylephrine in live cells of the aquatic pathogen Vibrio campbellii . Consequently, we have discovered the coupling protein CheW, which is in the center of the chemotaxis signaling network, as a target of both molecules. We not only demonstrate direct ligand binding to CheW but also elucidate how this affects chemotactic control. These findings are pivotal for further research on hormone-specific effects on bacterial behavior.

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Metadata

Authors
Weigert Muñoz, A., Hoyer, E., Schumacher, K., Grognot, M., Taute, K. M., Hacker, S. M., Sieber, S. A., & Jung, K.
Affiliations
  • Center for Functional Protein Assemblies, Technical University of Munich, 85748 Garching, Germany
  • Ludwig-Maximilians-Universität München (DE)
  • Harvard University (US)
  • Leiden University (NL)
  • Helmholtz Institute for Pharmaceutical Research Saarland (DE)
Corresponding author
  • Sieber, Stephan A.
  • Jung, Kirsten
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2022-03
Volume
119
Issue
10
Pages
e2118227119-e2118227119
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.2118227119
PMC
PMC8915975
OpenAlex
W4214923061
Cited by
16
References
4
Citations by year
2026: 3 · 2025: 1 · 2024: 5 · 2023: 5 · 2022: 2
FWCI
1.96
Type
journal article
Language
en
Open access
Licence
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion, cc-by-nc-nd)
  • ChemRxiv (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Leiden Repository (Leiden University) (submittedVersion, cc-by-nc-nd)
  • mediaTUM (Technical University of Munich) (submittedVersion, other-oa)
  • Data Archiving and Networked Services (DANS) (submittedVersion, cc-by-nc-nd)
Funders
  • Fonds der Chemischen Industrie
  • Deutsche Forschungsgemeinschaft (SFB1371, 395357507)
  • Universiteit Leiden
  • Studienstiftung des Deutschen Volkes
  • Technische Universität München
  • Rowland Institute at Harvard
  • European Research Council
  • Horizon 2020
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Immunology and Microbiology › Microbiology › Vibrio bacteria research studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Keywords
Vibrio campbellii, CheW, epinephrine, phenylephrine, chemotaxis, chemotaxis signaling, ligand binding, host-pathogen interaction, bacterial behavior, alpha-adrenergic agonists
MeSH terms
Bacterial Proteins, Catecholamines, Catechols, Chemotactic Factors, Chemotaxis, Iron, Protein Binding, Vibrio, Molecular Probes, Signal Transduction, Proteomics

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