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Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold
Open accessAbstract
Isolated newt ( Notophthalmus viridescens ) chromosomes were studied by using micromechanical force measurement during nuclease digestion. Micrococcal nuclease and short-recognition-sequence blunt-cutting restriction enzymes first remove the native elastic response of, and then to go on to completely disintegrate, single metaphase newt chromosomes. These experiments rule out the possibility that the mitotic chromosome is based on a mechanically contiguous internal non-DNA (e.g., protein) “scaffold”; instead, the mechanical integrity of the metaphase chromosome is due to chromatin itself. Blunt-cutting restriction enzymes with longer recognition sequences only partially disassemble mitotic chromosomes and indicate that chromatin in metaphase chromosomes is constrained by isolated chromatin-crosslinking elements spaced by ≈15 kb.
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Citations
Cited by
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Stephens, A. D., Banigan, E. J., Adam, S. A., Goldman, R. D., & Marko, J. F. (2017) · Molecular Biology of the Cell · doi:10.1091/mbc.e16-09-0653 · Cited by 473
Self-citationSingle-molecule experiments in biological physics: methods and applications
Ritort, F. (2006) · Journal of Physics: Condensed Matter · doi:10.1088/0953-8984/18/32/r01 · Cited by 393
Chromatin Higher-order Structure and Dynamics
Woodcock, C. L., & Ghosh, R. P. (2010) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a000596 · Cited by 384
Cell cycle and age-related modulations of mouse chromosome stiffness
Liu, N., Qiang, W., Jordan, P. W., Marko, J. F., & Qiao, H. (2025) · eLife · doi:10.7554/elife.97403 · Cited by 3
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Metadata
- Authors
- Poirier, M. G., & Marko, J. F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2002-11-18
- Volume
- 99
- Issue
- 24
- Pages
- 15393-15397
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2164938051
- Cited by
- 174
- References
- 0
- FWCI
- 3.24
- Type
- journal article
- Language
- en
- Open access
- green
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