The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Distinction between signaling mechanisms in lipid rafts vs. caveolae
Open accessAbstract
The relative importance of lipid rafts vs. specialized rafts termed caveolae to influence signal transduction is not known. Here we show that in cells lacking caveolae, the dually acylated protein, endothelial nitric oxide synthase (eNOS), localizes to cholesterol-rich lipid raft domains of the plasma membrane. In these cells, expression of caveolin-1 (cav-1) stimulates caveolae biogenesis, promotes the interaction of cav-1 with eNOS, and the inhibition of NO release from cells. Interestingly, in cells where cav-1 does not drive caveolae assembly, despite equal levels of cav-1 and eNOS and localization of both proteins to raft domains of the plasmalemma, the physical interaction of eNOS with cav-1 is dramatically less resulting in less inhibition of NO release. Thus, cav-1 concentrated in caveolae, not in rafts, is in closer proximity to eNOS and is necessary for negative regulation of eNOS function, thereby providing the first clear example of spatial regulation of signaling in this organelle that is distinct from raft domains.
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“NOX2 expression has been also reported in other cell types including endothelial cells and adventitial fibroblasts (376, 382, 657, 714, 715, 736, 876) (FIGURE 9, Table 5).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [876]
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“The current paradigm indicates that macrophage NOX2 is more detrimental in the development of cardiovascular disease (395, 876), with endothelial NOX2 probably involved in the initiation, but not so much in the development of the disease, and a subsequent progress of the atherosclerotic lesion dependent on immune cells (243, 573, 976).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [876]
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“NOX4 is the most abundant isoform in ECs, and it is also present in VSMCs and adventitial fibroblasts (10, 265, 736, 832, 876, 1036).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [876]
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Nitric oxide synthases: regulation and function
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Metadata
- Authors
- Sowa, G., Pypaert, M., & Sessa, W. C.
- Affiliations
- Yale University (US)
- Corresponding author
- Sessa, William C.
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2001-11-13
- Volume
- 98
- Issue
- 24
- Pages
- 14072-14077
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC61169
- OpenAlex
- W2076606695
- Cited by
- 233
- References
- 31
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 4 · 2023: 3 · 2022: 5 · 2021: 7 · 2020: 8 · 2019: 5 · 2018: 4 · 2017: 7 · 2016: 4 · 2015: 7 · 2014: 4 · 2013: 3 · 2012: 13
- FWCI
- 6.89
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Heart, Lung, and Blood Institute (HL-61371, HL-64793, R01HL064793, R01HL057665, R01HL061371, R37HL061371)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Keywords
- lipid rafts, caveolae, caveolin-1, endothelial nitric oxide synthase, signal transduction, cholesterol, protein-protein interaction, nitric oxide release, plasma membrane, spatial regulation of signaling
- MeSH terms
- Animals, Cells, Cultured, Cholesterol, Humans, Nitric Oxide, Rats, Inbred F344, Thyroid Gland, Signal Transduction, Nitric Oxide Synthase, Caveolae, Membrane Microdomains, Caveolins, Caveolin 1, Rats, Nitric Oxide Synthase Type III
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