The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Noninvasive imaging of protein–protein interactions in living subjects by using reporter protein complementation and reconstitution strategies
Open accessAbstract
In this study we have developed bioluminescence-imaging strategies to noninvasively and quantitatively image protein—protein interactions in living mice by using a cooled charge-coupled device camera and split reporter technology. We validate both complementation and intein-mediated reconstitution of split firefly luciferase proteins driven by the interaction of two strongly interacting proteins, MyoD and Id. We use transient transfection of cells and image MyoD–Id interaction after induction of gene expression in cell culture and in cells implanted into living mice. Techniques to study protein–protein interactions in living subjects will allow the study of cellular networks, including signal transduction pathways, as well as development and optimization of pharmaceuticals for modulating protein–protein interactions.
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Iyer, M., Wu, L., Carey, M., Wang, Y., Smallwood, A., & Gambhir, S. S. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.251551098 · Cited by 227
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Cited by
Kerppola, T. K. (2008) · Annual Review of Biophysics · doi:10.1146/annurev.biophys.37.032807.125842 · Cited by 709
Applications of bioluminescence in biotechnology and beyond
Syed, A. J., & Anderson, J. C. (2021) · Chemical Society Reviews · doi:10.1039/d0cs01492c · Cited by 341
Paulmurugan, R., & Gambhir, S. S. (2003) · Analytical Chemistry · doi:10.1021/ac020731c · Cited by 310
Self-citationVery long-term memories may be stored in the pattern of holes in the perineuronal net
Tsien, R. Y. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1310158110 · Cited by 244
Dragulescu-Andrasi, A., Chan, C. T., De, A., Massoud, T. F., & Gambhir, S. S. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1100923108 · Cited by 186
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Metadata
- Authors
- Paulmurugan, R., Umezawa, Y., & Gambhir, S. S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2002-11-18
- Volume
- 99
- Issue
- 24
- Pages
- 15608-15613
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1620954807
- Cited by
- 334
- References
- 1
- FWCI
- 7.34
- Type
- journal article
- Language
- en
- Open access
- green
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- Paulmurugan, R., Umezawa, Y., & Gambhir, S. S. (2002). Noninvasive imaging of protein–protein interactions in living subjects by using reporter protein complementation and reconstitution strategies. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.242594299
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