Clonal growth of mammalian cells in a chemically defined, synthetic medium
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Basal media such as DMEM, F-12, or DMEM/F-12 combinations are inherently xeno-free, protein-free, and chemically defined.
“Basal media such as Dulbecco’s Modified Eagle Medium (DMEM), Ham’s F-12 (F-12), or DMEM/F-12 combinations are inherently xeno-free, protein-free, and chemically defined (Eagle, 1959; Ham, 1965).”
From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Ham, 1965
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Research works printed but not linked: 19
- 1 Puck, T. T., P. I. Marcus, and S. J. Cieciura, J. Exptl. Med., 103, 273 (1956).
- 2 Fisher, H. W., T. T. Puck, and G. Sato, J. Exptl. Med., 109, 649 (1959).
- 3 Gwatkin, R. B. L., Nature, 186, 984 (1960).
- 4 Ham, R. G., Exptl. Cell Res., 28, 489 (1962).
- Ibid., 29, 515 (1963).
- ' Ham, R. G., Science, 140, 802 (1963).
- 7 Ham, R. G., Biochem. Biophys. Res. Commun. 14, 34 (1964).
- 8 Puck, T. T., S. J. Cieciura, and A. Robinson, J. Exptl. Med., 108, 945 (1958).
- Ham, R. G., and T. T. Puck, in Methods in Enzymology, ed. S. P. Colowick and N. O. Kaplan (New York: Academic Press, 1962), vol. 5, p. 90.
- 10 Healey, G. M., D. C. Fisher, and R. C. Parker, Proc. Soc. Exptl. Biol. Med., 89, 71 (1955).
- 12 Waymouth, C., J. Natl. Cancer Inst., 22, 1003 (1959).
- 13 Katsuta, H., and T. Takaoka, Japan. J. Exptl. Med., 30, 235 (1960).
- 14 Merchant, D. J., and K. B. Hellman, Proc. Soc. Exptl. Biol. Med., 110, 194 (1962).
- 16 Fioramonti, M. C., V. J. Evans, and W. R. Earle, J. Natl. Cancer Inst., 21, 579 (1958).
- 1 Sanford, K. K., L. T. Dupree, and A. B. Covalesky, Exptl. Cell Res., 31, 345 (1963).
- 17 Sabina, L. R., A. L. Tosoni, and R. C. Parker, Proc. Soc. Exptl. Biol. Med., 114, 13 (1963).
- 18 Evans, V. J., H. A. Kerr, W. T. McQuilkin, W. R. Earle, and R. N. Hull, Am. J. Hygiene, 70, 297 (1959).
- 19 Katsuta, H., T. Takaoka, and K. Kikuchi, Japan. J. Exptl. Med., 31, 125 (1961).
- 20 Alarcon, R. A., Arch. Biochem. Biophys., 106, 240 (1964).
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Chasin, L. A., Feldman, A., Konstam, M., & Urlaub, G. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.3.718 · Cited by 81 · see citing works
Spänle, J. V., Haiber, L. M., & Seeger, B. (2026) · Frontiers in Toxicology · doi:10.3389/ftox.2026.1800157 · Cited by 1 · see citing works
- Authors
- Ham, R. G.
- Affiliations
- DEPARTMENT OF BIOPHYSICS, UNIVERSITY OF COLORADO MEDICAL CENTER
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1965-02
- Volume
- 53
- Issue
- 2
- Pages
- 288-293
- ISSN
- 0027-8424
- 1091-6490
- Cited by
- 559
- References
- 0
- Language
- en
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