The DOI matches its Crossref record.
FORMATION AND DETECTION OF RNA-DNA HYBRID MOLECULES IN CYTOLOGICAL PREPARATIONS
Open accessAbstract
A technique is described for forming molecular hybrids between RNA in solution and the DNA of intact cytological preparations. Cells in a conventional tissue squash are immobilized under a thin layer of agar. Next they are treated with alkali to denature the DNA and then incubated with tritium-labeled RNA. The hybrids are detected by autoradiography. The technique is illustrated by the hybridization of ribosomal RNA to the amplified ribosomal genes in oocytes of the toad Xenopus . A low level of gene amplification was also detected in premeiotic nuclei (oogonia).
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Citations
Cited by
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DeLong, E. F., Wickham, G. S., & Pace, N. R. (1989) · Science · doi:10.1126/science.2466341 · Cited by 1,238
Chromosomal Localization of Mouse Satellite DNA
Pardue, M. L., & Gall, J. G. (1970) · Science · doi:10.1126/science.168.3937.1356 · Cited by 1,119
Self-citationCRISPR/Cas9 in Genome Editing and Beyond
Wang, H., La Russa, M., & Qi, L. S. (2016) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060815-014607 · Cited by 1,110
Metadata
- Authors
- Gall, J. G., & Pardue, M. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1969-06
- Volume
- 63
- Issue
- 2
- Pages
- 378-383
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2049046722
- Cited by
- 1,274
- References
- 0
- FWCI
- 42.29
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: FORMATION AND DETECTION OF RNA-DNA HYBRID MOLECULES IN CYTOLOGICAL PREPARATIONS. https://app.cochat.ai/papers/10.1073/pnas.63.2.378
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- Gall, J. G., & Pardue, M. L. (1969). FORMATION AND DETECTION OF RNA-DNA HYBRID MOLECULES IN CYTOLOGICAL PREPARATIONS. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.63.2.378
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
