The DOI matches its Crossref record.
Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing Lambda Phage Genes and the Galactose Operon of Escherichia coli
Open accessAbstract
We have developed methods for covalently joining duplex DNA molecules to one another and have used these techniques to construct circular dimers of SV40 DNA and to insert a DNA segment containing lambda phage genes and the galactose operon of E. coli into SV40 DNA. The method involves: ( a ) converting circular SV40 DNA to a linear form, ( b ) adding single-stranded homodeoxypolymeric extensions of defined composition and length to the 3′ ends of one of the DNA strands with the enzyme terminal deoxynucleotidyl transferase ( c ) adding complementary homodeoxypolymeric extensions to the other DNA strand, ( d ) annealing the two DNA molecules to form a circular duplex structure, and ( e ) filling the gaps and sealing nicks in this structure with E. coli DNA polymerase and DNA ligase to form a covalently closed-circular DNA molecule.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
A vision for the future of genomics research
Collins, F. S., Green, E. D., Guttmacher, A. E., Guyer, M. S., & on behalf of the US National Human Genome Research Institute (2003) · Nature · doi:10.1038/nature01626 · Cited by 1,752
The sequence of sequencers: The history of sequencing DNA
Heather, J. M., & Chain, B. (2016) · Genomics · doi:10.1016/j.ygeno.2015.11.003 · Cited by 1,397
“The development of techniques such as polymerase chain reaction (PCR) [37,38] and recombinant DNA technologies[39,40]further aided the genomicsrevolution by pro-vidingmeans of generatingthe highconcentrationsof pure DNAspecies required for sequencing.”
MethodTransformation of yeast by a replicating hybrid plasmid
Beggs, J. D. (1978) · Nature · doi:10.1038/275104a0 · Cited by 1,316
Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts
Stewart, M. P., Langer, R., & Jensen, K. F. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00678 · Cited by 747
Historical perspective, development and applications of next-generation sequencing in plant virology
Barba, M., Czosnek, H., & Hadidi, A. (2014) · Viruses · doi:10.3390/v6010106 · Cited by 317
Metadata
- Authors
- Jackson, D. A., Symons, R. H., & Berg, P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1972-10
- Volume
- 69
- Issue
- 10
- Pages
- 2904-2909
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2136451426
- Cited by
- 778
- References
- 0
- FWCI
- 6.83
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing Lambda Phage Genes and the Galactose Operon of Escherichia coli. https://app.cochat.ai/papers/10.1073/pnas.69.10.2904
- The paper
- Jackson, D. A., Symons, R. H., & Berg, P. (1972). Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing Lambda Phage Genes and the Galactose Operon of Escherichia coli. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.69.10.2904
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
