The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose
Open accessAbstract
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active rabbit globin mRNA has been purified by this procedure and assayed for its ability to direct the synthesis of rabbit globin in a cell-free extract of ascites tumor. Inasmuch as various mammalian mRNAs appear to be rich in poly(adenylic acid) and can likely be translated in the ascites cell-free extract, this approach should prove generally useful as an initial step in the isolation of specific mRNAs.
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Cites
Edmonds, M., Vaughan, M. H., & Nakazato, H. (1971) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.68.6.1336 · Cited by 510
In Vitro Synthesis of DNA Complementary to Purified Rabbit Globin mRNA
Ross, J., Aviv, H., Scolnick, E., & Leder, P. (1972) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.69.1.264 · Cited by 192
Self-citation
Cited by
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J., & Rutter, W. J. (1979) · Biochemistry · doi:10.1021/bi00591a005 · Cited by 21,725
Identification of the Cystic Fibrosis Gene: Cloning and Characterization of Complementary DNA
Riordan, J. R., Rommens, J. M., Kerem, B.-S., Alon, N., Rozmahel, R., Grzelczak, Z., Zielenski, J., Lok, S., Plavsic, N., Chou, J.-L., et al. (1989) · Science · doi:10.1126/science.2475911 · Cited by 7,418
The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor
Kang, J., Lemaire, H.-G., Unterbeck, A., Salbaum, J. M., Masters, C. L., Grzeschik, K.-H., Multhaup, G., Beyreuther, K., & Müller-Hill, B. (1987) · Nature · doi:10.1038/325733a0 · Cited by 4,619
Verkerk, A. J. M. H., Pieretti, M., Sutcliffe, J. S., Fu, Y.-H., Kuhl, D. P. A., Pizzuti, A., Reiner, O., Richards, S., Victoria, M. F., Zhang, F., et al. (1991) · Cell · doi:10.1016/0092-8674(91)90397-h · Cited by 3,481
Heinzel, F. P., Sadick, M. D., Holaday, B. J., Coffman, R. L., & Locksley, R. M. (1989) · The Journal of experimental medicine · doi:10.1084/jem.169.1.59 · Cited by 1,454
Metadata
- Authors
- Aviv, H., & Leder, P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1972-06
- Volume
- 69
- Issue
- 6
- Pages
- 1408-1412
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2066160869
- Cited by
- 6,751
- References
- 2
- FWCI
- 34.17
- Type
- journal article
- Language
- en
- Open access
- green
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- Aviv, H., & Leder, P. (1972). Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.69.6.1408
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