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Cited by 6,751

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

Open access
Aviv, Haim, Leder, Philip·1972·Proceedings of the National Academy of Sciences

Abstract

A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active rabbit globin mRNA has been purified by this procedure and assayed for its ability to direct the synthesis of rabbit globin in a cell-free extract of ascites tumor. Inasmuch as various mammalian mRNAs appear to be rich in poly(adenylic acid) and can likely be translated in the ascites cell-free extract, this approach should prove generally useful as an initial step in the isolation of specific mRNAs.

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Crossref · 10.1073/pnas.69.6.1408

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Metadata

Authors
Aviv, H., & Leder, P.
Venue
Proceedings of the National Academy of Sciences
Published
1972-06
Volume
69
Issue
6
Pages
1408-1412
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2066160869
Cited by
6,751
References
2
FWCI
34.17
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose. https://app.cochat.ai/papers/10.1073/pnas.69.6.1408
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Aviv, H., & Leder, P. (1972). Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.69.6.1408

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.