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Comparison of Nucleolar Proteins of Normal Rat Liver and Novikoff Hepatoma Ascites Cells by Two-Dimensional Polyacrylamide Gel Electrophoresis
Open accessAbstract
A two-dimensional polyacrylamide gel electrophoresis technique is presented for separation of acid-extracted proteins of isolated nucleoli of normal rat liver and Novikoff hepatoma ascites cells. About 100 distinct protein spots were resolved. Comparison of the patterns for normal liver and Novikoff hepatoma revealed nine spots with markedly different intensities in the two patterns. Two spots were found that were unique to the normal liver pattern, and one spot was found that was unique to the hepatoma pattern.
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Citations
Cited by
High resolution two-dimensional electrophoresis of proteins.
O'Farrell, P. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)41496-8 · Cited by 19,246
Lapeyre, B., Bourbon, H., & Amalric, F. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.6.1472 · Cited by 386
Therapeutic applications of AS1411 aptamer, an update review
Yazdian-Robati, R., Bayat, P., Oroojalian, F., Zargari, M., Ramezani, M., Taghdisi, S. M., & Abnous, K. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.11.118 · Cited by 288
Isolation and characterization of protein A24, a "histone-like" non-histone chromosomal protein.
Goldknopf, I. L., Taylor, C. W., Baum, R. M., Yeoman, L. C., Olson, M. O., Prestayko, A. W., & Busch, H. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)40926-5 · Cited by 278
Self-citationCeman, S., Brown, V., & Warren, S. T. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.12.7925 · Cited by 181
Metadata
- Authors
- Orrick, L. R., Olson, M. O. J., & Busch, H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1973-05
- Volume
- 70
- Issue
- 5
- Pages
- 1316-1320
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2011364690
- Cited by
- 267
- References
- 0
- FWCI
- 10.25
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Comparison of Nucleolar Proteins of Normal Rat Liver and Novikoff Hepatoma Ascites Cells by Two-Dimensional Polyacrylamide Gel Electrophoresis. https://app.cochat.ai/papers/10.1073/pnas.70.5.1316
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- Orrick, L. R., Olson, M. O. J., & Busch, H. (1973). Comparison of Nucleolar Proteins of Normal Rat Liver and Novikoff Hepatoma Ascites Cells by Two-Dimensional Polyacrylamide Gel Electrophoresis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.70.5.1316
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
