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Methylation of Newly Synthesized Viral Messenger RNA by an Enzyme in Vaccinia Virus
Abstract
Purified vaccinia virions contain an enzyme that incorporates methyl groups from S -adenosylmethionine into viral RNA synthesized by the core-associated DNA-dependent RNA polymerase. This incorporation, by partially disrupted virions, was dependent on the presence of all four ribonucleoside triphosphates and Mg ++ and was inhibited by actinomycin D. At saturation, 2.3 methyl groups were incorporated per 1000 nucleotides. The methyl-labeled RNA product was sensitive to alkali and ribonucleases and hybridized to filters containing immobilized poly(U) or vaccinia DNA. The methyl groups were not located on the 3′-terminal polyadenylate sequence, nor were they randomly distributed along the RNA chain. The lability of a large portion of the methyl groups to perchloric acid digestion was consistent with an O-methyl linkage, and the chromatographic properties of the alkali-digested material suggested that either the 5′-terminus or up to three consecutive internal nucleotides were methylated. Methylation probably occurs at the macromolecular level, since added vaccinia RNA was a suitable substrate. The failure of heterologous rRNA and tRNA species as well as homopolyribonucleotides to act as substrate suggested that a specific sequence might be required.
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“Methyltransferase like 3 (METTL3) was the first methyltransferase implicated in placing m6A on RNA”
From HNRNPA2B1 Is a Mediator of m6A-Dependent Nuclear RNA Processing Events (2015) · cites this paper as Wei and Moss, 1974
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Cited by
HNRNPA2B1 Is a Mediator of m6A-Dependent Nuclear RNA Processing Events
Alarcón, C. R., Goodarzi, H., Lee, H., Liu, X., Tavazoie, S., & Tavazoie, S. F. (2015) · Cell · doi:10.1016/j.cell.2015.08.011 · Cited by 1,514
m6A RNA Methylation Regulates the Self-Renewal and Tumorigenesis of Glioblastoma Stem Cells
Cui, Q., Shi, H., Ye, P., Li, L., Qu, Q., Sun, G., Sun, G., Lu, Z., Huang, Y., Yang, C.-G., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.02.059 · Cited by 1,252
Metadata
- Authors
- Wei, C. M., & Moss, B.
- Affiliations
- Laboratory of Biology of Viruses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1974-08
- Volume
- 71
- Issue
- 8
- Pages
- 3014-3018
- ISSN
- 0027-8424
- 1091-6490
- Cited by
- 65
- References
- 2
- Language
- en
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