The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
Open accessAbstract
A method has been developed whereby a very large number of colonies of Escherichia coli carrying different hybrid plasmids can be rapidly screened to determine which hybrid plasmids contain a specified DNA sequence or genes. The colonies to be screened are formed on nitrocellulose filters, and, after a reference set of these colonies has been prepared by replica plating, are lysed and their DNA is denatured and fixed to the filter in situ. The resulting DNA-prints of the colonies are then hybridized to a radioactive RNA that defines the sequence or gene of interest, and the result of this hybridization is assayed by autoradiography. Colonies whose DNA-prints exhibit hybridization can then be picked from the reference plate. We have used this method to isolate clones of ColE1 hybrid plasmids that contain Drosophila melanogaster genes for 18 and 28S rRNAs. In principle, the method can be used to isolate any gene whose base sequence is represented in an available RNA.
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Cites
Plasmid ColE1 as a Molecular Vehicle for Cloning and Amplification of DNA
Hershfield, V., Boyer, H. W., Yanofsky, C., Lovett, M. A., & Helinski, D. R. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.9.3455 · Cited by 540
Katz, L., Kingsbury, D. T., & Helinski, D. R. (1973) · Journal of Bacteriology · doi:10.1128/jb.114.2.577-591.1973 · Cited by 496
A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster
Wensink, P. (1974) · Cell · doi:10.1016/0092-8674(74)90045-2 · Cited by 476
Glover, D. M., White, R. L., Finnegan, D., & Hogness, D. S. (1975) · Cell · doi:10.1016/0092-8674(75)90023-9 · Cited by 131
Self-citation
Cited by
Transformation of yeast by a replicating hybrid plasmid
Beggs, J. D. (1978) · Nature · doi:10.1038/275104a0 · Cited by 1,316
Natural resistance to infection with intracellular parasites: isolation of a candidate for Bcg
Vidal, S. M., Malo, D., Vogan, K., Skamene, E., & Gros, P. (1993) · Cell · doi:10.1016/0092-8674(93)90135-d · Cited by 1,104
Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis
Mahairas, G. G., Sabo, P. J., Hickey, M. J., Singh, D. C., & Stover, C. K. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.5.1274-1282.1996 · Cited by 1,103
Linkage map of Escherichia coli K-12, edition 6
Bachmann, B., & Low, K. B. (1980) · Microbiological Reviews · doi:10.1128/mmbr.44.1.1-56.1980 · Cited by 938
Comparative biochemical properties of normal and activated human ras p21 protein
McGrath, J. P., Capon, D. J., Goeddel, D. V., & Levinson, A. D. (1984) · Nature · doi:10.1038/310644a0 · Cited by 699
Metadata
- Authors
- Grunstein, M., & Hogness, D. S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1975-10
- Volume
- 72
- Issue
- 10
- Pages
- 3961-3965
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2040276985
- Cited by
- 3,431
- References
- 4
- FWCI
- 18.96
- Type
- journal article
- Language
- en
- Open access
- green
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- Grunstein, M., & Hogness, D. S. (1975). Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.72.10.3961
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