The DOI matches its Crossref record.
O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.
Open accessAbstract
O-Phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products. Picomole quantities of amino acids, peptides, and proteins can be detected easily. o-Phthalaldehyde is five to ten times more sensitive than fluorescamine and is soluble and stable in aqueous buffers.
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Cited by
Glomb, M. A., & Monnier, V. M. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.17.10017 · Cited by 576
Permeability of small nonelectrolytes through lipid bilayer membranes
Walter, A., & Gutknecht, J. (1986) · The Journal of Membrane Biology · doi:10.1007/bf01870127 · Cited by 502
Metadata
- Authors
- Benson, J. R., & Hare, P. E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1975-02
- Volume
- 72
- Issue
- 2
- Pages
- 619-622
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1990633912
- Cited by
- 737
- References
- 0
- FWCI
- 20.33
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
