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Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.

Open access
Pribnow, D·1975·Proceedings of the National Academy of Sciences

Abstract

Escherichia coli RNA polymerase (EC 2.7.7.6), bound in a tight complex at an early T7 promoter, protects 41 to 43 base pairs of DNA from digestion by DNase. I. The protected DNA fragment contains both the binding site for RNA polymerase and the mRNA initiation point for the promoter. The sequence of the DNA fragment and the sequence of the mRNA that it codes for are presented here. A seven-base-pair sequence, apparently common to all promoters, is implicated in the formation of a tight binary complex with RNA polymerase.

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Crossref · 10.1073/pnas.72.3.784

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Metadata

Authors
Pribnow, D.
Venue
Proceedings of the National Academy of Sciences
Published
1975-03
Volume
72
Issue
3
Pages
784-788
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2039555609
Cited by
606
References
0
FWCI
19.87
Type
journal article
Language
en
Open access
green

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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