The DOI matches its Crossref record.
Growth of mouse megakaryocyte colonies in vitro.
Open accessAbstract
Mouse bone marrow and spleen cells formed pure or mixed colonies of up to 80 megakaryocytes in agar cultures after stimulation by medium conditioned by activated mouse lymphoid cells. Megakaryocytes were identified on the basis of their morphology, polyploid mitoses and DNA content, and high cytoplasmic content of acetylcholinesterase. Megakaryocyte colony-forming cells were relatively small with a peak sedimentation velocity of 4.2 mm/hr. Spleen, lymph node, and thymus cells produced the factor stimulating megakaryocyte proliferation after culture in medium containing 2-mercaptoethanol, with or without added mitogens or allogeneic spleen cells. Peak activity in conditioning medium was associated with the small lymphocyte fractions in mouse spleen.
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Citations
Cited by
Dick, J. E., Magli, M. C., Huszar, D., Phillips, R. A., & Bernstein, A. (1985) · Cell · doi:10.1016/s0092-8674(85)80102-1 · Cited by 446
Metcalf, D., Johnson, G., & Burgess, A. (1980) · Blood · doi:10.1182/blood.v55.1.138.138 · Cited by 176
Self-citationDexter, T. M., Spooncer, E., Toksoz, D., & Lajtha, L. G. (1980) · Journal of Supramolecular Structure · doi:10.1002/jss.400130410 · Cited by 81
Metadata
- Authors
- Metcalf, D., MacDonald, H. R., Odartchenko, N., & Sordat, B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1975-05
- Volume
- 72
- Issue
- 5
- Pages
- 1744-1748
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2030955918
- Cited by
- 283
- References
- 0
- FWCI
- 17.61
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Growth of mouse megakaryocyte colonies in vitro. https://app.cochat.ai/papers/10.1073/pnas.72.5.1744
- The paper
- Metcalf, D., MacDonald, H. R., Odartchenko, N., & Sordat, B. (1975). Growth of mouse megakaryocyte colonies in vitro. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.72.5.1744
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
