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Cited by 329

The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.

Characterization of a common precursor to corticotropin and beta-lipotropin: identification of beta-lipotropin peptides and their arrangement relative to corticotropin in the precursor synthesized in a cell-free system.

Open access
Roberts, J L, Herbert, E·1977·Proceedings of the National Academy of Sciences

Abstract

Radioactive proteins synthesized in an mRNA-dependent reticulocyte cell-free system under the direction of mRNA from AtT-20/D-16v mouse cells were isolated by specific immunoprecipitation using antiserum to either alpha(1-24) corticotropin or beta-endorphin [beta(61-91) lipotropin]. Each immunoprecipitate was fractionated by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and shown to contain only one labeled protein with an apparent molecular weight of 28,500. Tryptic peptide analysis of the Mr 28,500 corticotropin and beta-lipotropin molecules isolated from the gels demonstrated that the two proteins had the same lysine, methionine, and tryptophan peptides. Four tryptic peptides from the cell-free product exhibited the same electrophoretic and chromatographic mobilities as marker tryptic peptides from bovine beta-melanotropin and porcine beta-endorphin. The identification of these peptides was confirmed by amino acid composition studies with a variety of labeled amino acids. The beta-lipotropin tryptic peptides were also shown to be located carboxy terminal to the corticotropin tryptic peptides.

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Metadata

Authors
Roberts, J. L., & Herbert, E.
Venue
Proceedings of the National Academy of Sciences
Published
1977-12
Volume
74
Issue
12
Pages
5300-5304
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2050318725
Cited by
329
References
13
FWCI
15.02
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Characterization of a common precursor to corticotropin and beta-lipotropin: identification of beta-lipotropin peptides and their arrangement relative to corticotropin in the precursor synthesized in a cell-free system. https://app.cochat.ai/papers/10.1073/pnas.74.12.5300
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Roberts, J. L., & Herbert, E. (1977). Characterization of a common precursor to corticotropin and beta-lipotropin: identification of beta-lipotropin peptides and their arrangement relative to corticotropin in the precursor synthesized in a cell-free system. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.74.12.5300

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