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Specific radiolabeling of a cell surface receptor for epidermal growth factor.
Open accessAbstract
A photoreactive derivative of epidermal growth factor (EGF) has been used to identify and specifically label a membrane receptor for EGF on mouse 3T3 cells. Photoactivable EGF, labeled with 125I, was incubated with 3T3 cells and then photolyzed in situ to generate a nitrene capable of reacting with a wide variety of chemical bonds. Analysis of the system by sodium dodecyl sulfate/polyacrylamide gel electrophoresis revealed, besides the band of EGF, only one other major radioactive band, at a position indicating an apparent molecular weight of 190,000. This band was absent when a nonresponsive and nonbinding variant of 3T3 was used. A direct proportionality between binding activity and crosslinked complex formation was demonstrated using a variety of binding conditions. "Down regulated" cells, in which EGF binding activity was greatly reduced by prolonged incubation with an appropriate concentration of EGF, also had a decrease in covalent complex formation proportional to the decrease in EGF binding activity.
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Citations
Cited by
Growth factors: Mechanism of action and relation to oncogenes
Heldin, C.-H., & Westermark, B. (1984) · Cell · doi:10.1016/0092-8674(84)90296-4 · Cited by 854
The platelet-derived growth factor
Ross, R., & Vogel, A. (1978) · Cell · doi:10.1016/0092-8674(78)90107-1 · Cited by 640
Epidermal growth factor receptors
Adamson, E. D., & Rees, A. R. (1981) · Molecular and Cellular Biochemistry · doi:10.1007/bf02359619 · Cited by 349
Epidermal growth factor. Relationship between receptor regulation and mitogenesis in 3T3 cells.
Aharonov, A., Pruss, R. M., & Herschman, H. R. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34785-3 · Cited by 324
Self-citationMaking the first move in EGFR-driven or ALK-driven NSCLC: first-generation or next-generation TKI?
Recondo, G., Facchinetti, F., Olaussen, K. A., Besse, B., & Friboulet, L. (2018) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-018-0081-4 · Cited by 321
Metadata
- Authors
- Das, M., Miyakawa, T., Fox, C. F., Pruss, R. M., Aharonov, A., & Herschman, H. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1977-07
- Volume
- 74
- Issue
- 7
- Pages
- 2790-2794
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1969123562
- Cited by
- 206
- References
- 0
- FWCI
- 16.53
- Type
- journal article
- Language
- en
- Open access
- green
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- Das, M., Miyakawa, T., Fox, C. F., Pruss, R. M., Aharonov, A., & Herschman, H. R. (1977). Specific radiolabeling of a cell surface receptor for epidermal growth factor. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.74.7.2790
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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