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Detection of prokaryotic signal peptidase in an Escherichia coli membrane fraction: endoproteolytic cleavage of nascent f1 pre-coat protein.
Open accessAbstract
An inverted membrane vesicle fraction isolated from uninfected Escherichia coli and largely derived from the inner membrane has been shown to contain an endoproteolytic activity that cleaves nascent bacteriophage f1 pre-coat protein into two identifiable products. The electrophoretic mobility on sodium dodecyl sulfate/urea/polyacrylamide gels and the partial amino-terminal sequence of the larger fragment were indistinguishable from those of the mature phage coat protein. Partial amino-terminal sequence analysis showed that the smaller fragment corresponds to the amino-terminal "signal peptide" of f1 pre-coat protein. Cleavage occurred only if the membrane fraction was present during in vitro synthesis, and was not observed if it was added after completion of pre-coat protein synthesis. The cleavage reaction was strongly stimulated when the membrane fraction was present together with the nonionic detergent Nikkol. These results are consistent with and discussed in terms of the signal hyothesis.
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Cited by
Hartl, F.-U., Lecker, S., Schiebel, E., Hendrick, J. P., & Wickner, W. (1990) · Cell · doi:10.1016/0092-8674(90)90160-g · Cited by 562
Lill, R., Dowhan, W., & Wickner, W. (1990) · Cell · doi:10.1016/0092-8674(90)90742-w · Cited by 550
ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocase
Schiebel, E., Driessen, A. J. M., Hartl, F.-U., & Wickner, W. (1991) · Cell · doi:10.1016/0092-8674(91)90317-r · Cited by 458
Paetzel, M., Karla, A., Strynadka, N. C. J., & Dalbey, R. E. (2002) · Chemical Reviews · doi:10.1021/cr010166y · Cited by 440
Secretion and Membrane Localization of Proteins in Escherichia Coli
Inouye, M., Halegoua, S., & Beckwith, J. (1980) · Critical Reviews in Biochemistry · doi:10.3109/10409238009105465 · Cited by 401
Metadata
- Authors
- Chang, C. N., Blobel, G., & Model, P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1978-01
- Volume
- 75
- Issue
- 1
- Pages
- 361-365
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2042826426
- Cited by
- 209
- References
- 0
- FWCI
- 22.01
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Detection of prokaryotic signal peptidase in an Escherichia coli membrane fraction: endoproteolytic cleavage of nascent f1 pre-coat protein. https://app.cochat.ai/papers/10.1073/pnas.75.1.361
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- Chang, C. N., Blobel, G., & Model, P. (1978). Detection of prokaryotic signal peptidase in an Escherichia coli membrane fraction: endoproteolytic cleavage of nascent f1 pre-coat protein. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.75.1.361
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