The DOI matches its Crossref record.
O4-(5'-uridylyl)tyrosine is the bond between the genome-linked protein and the RNA of poliovirus.
Open accessAbstract
Virion RNA of poliovirus type 1 has been analyzed for the linkage between genome-protein VPg and the polyribonucleotide chain. Hydrolysis of the linkage with acid or alkali and enzymatic degradation lead to the conclusion that the bond is neither a phosphodiester such as nucleotidyl-(P-O)-serine (or threonine) nor a phosphoramidate such as nucleotidyl-(P-N)-amino acid. VPg-RNA can be iodinated by the Bolton and Hunter reagent [iodinated 3-(4-hydroxyphenyl)propionic acid N-hydroxysuccinimide ester] but not by the chloramine-T or lactoperoxidase procedures, an observation suggesting that VPg does not contain accessible tyrosine. However, VPg can be labeled with [3H]tyrosine in vivo. Hydrolysis of VPg-[32P]pUp with 5.6 M HCl at 110 degrees yielded 32P-labeled O4-(3'-phospho-5'-uridylyl)tyrosine that could be cleaved with micrococcal nuclease to O4-[32P]phosphotyrosine and uridine 3'-[32P]phosphate. These data establish that VPg is linked to the poliovirus genome by a bond between the O4 of tyrosine and the 5'-P atom of the terminal uridylic acid residue. The 5' end of polio genome RNA can now be described as VPg(Tyr-O)-pU-U-A-A-A-A-C-A-G.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Transforming gene product of Rous sarcoma virus phosphorylates tyrosine
Hunter, T., & Sefton, B. M. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.3.1311 · Cited by 2,332
Primary structure, gene organization and polypeptide expression of poliovirus RNA
Kitamura, N., Semler, B. L., Rothberg, P. G., Larsen, G. R., Adler, C. J., Dorner, A. J., Emini, E. A., Hanecak, R., Lee, J. J., van der Werf, S., et al. (1981) · Nature · doi:10.1038/291547a0 · Cited by 914
Self-citationStimulation of tyrosine-specific phosphorylation by platelet-derived growth factor
Ek, B., Westermark, B., Wasteson, Å., & Heldin, C.-H. (1982) · Nature · doi:10.1038/295419a0 · Cited by 658
Mechanism of action of a yeast RNA ligase in tRNA splicing
Greer, C. L., Peebles, C. L., Gegenheimer, P., & Abelson, J. (1983) · Cell · doi:10.1016/0092-8674(83)90473-7 · Cited by 311
Argos, P., Kamer, G., Nicklin, M. J. H., & Wimmer, E. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.18.7251 · Cited by 255
Self-citation
Metadata
- Authors
- Rothberg, P. G., Harris, T. J., Nomoto, A., & Wimmer, E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1978-10
- Volume
- 75
- Issue
- 10
- Pages
- 4868-4872
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2151062650
- Cited by
- 249
- References
- 0
- FWCI
- 16.70
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: O4-(5'-uridylyl)tyrosine is the bond between the genome-linked protein and the RNA of poliovirus. https://app.cochat.ai/papers/10.1073/pnas.75.10.4868
- The paper
- Rothberg, P. G., Harris, T. J., Nomoto, A., & Wimmer, E. (1978). O4-(5'-uridylyl)tyrosine is the bond between the genome-linked protein and the RNA of poliovirus. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.75.10.4868
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
