The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A bacterial clone synthesizing proinsulin.
Open accessAbstract
We have cloned double-stranded cDNA copies of a rat preproinsulin messenger RNA in Escherichia coli chi1776, using the unique Pst endonuclease site of plasmid pBR322 that lies in the region encoding amino acids 181-182 of penicillinase. This site was reconstructed by inserting the cDNA with an oligo(dG)-oligo(dC) joining procedure. One of the clones expresses a fused protein bearing both insulin and penicillinase antigenic determinants. The DNA sequence of this plasmid shows that the insulin region is read in phase; a stretch of six glycine residues connects the alanine at position 182 of penicillinase to the fourth amino acid, glutamine, of rat proinsulin.
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Citations
Cites
Gilbert, W. (1978) · Nature · doi:10.1038/271501a0 · Cited by 2,300
Self-citation
Cited by
Plasmid screening at high colony density
Hanahan, D., & Meselson, M. (1980) · Gene · doi:10.1016/0378-1119(80)90144-4 · Cited by 1,078
A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification
Hopp, T. P., Prickett, K. S., Price, V. L., Libby, R. T., March, C. J., Pat Cerretti, D., Urdal, D. L., & Conlon, P. J. (1988) · Bio/Technology · doi:10.1038/nbt1088-1204 · Cited by 957
A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
Okayama, H., & Berg, P. (1983) · Molecular and Cellular Biology · doi:10.1128/mcb.3.2.280 · Cited by 933
Ravetch, J. V., Siebenlist, U., Korsmeyer, S., Waldmann, T., & Leder, P. (1981) · Cell · doi:10.1016/0092-8674(81)90400-1 · Cited by 930
The purification of eukaryotic polypeptides synthesized in Escherichia coli
Marston, F. A. (1986) · Biochemical Journal · doi:10.1042/bj2400001 · Cited by 836
Metadata
- Authors
- Villa-Komaroff, L., Efstratiadis, A., Broome, S., Lomedico, P., Tizard, R., Naber, S. P., Chick, W. L., & Gilbert, W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1978-08
- Volume
- 75
- Issue
- 8
- Pages
- 3727-3731
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2012880496
- Cited by
- 510
- References
- 1
- FWCI
- 43.74
- Type
- journal article
- Language
- en
- Open access
- green
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- Villa-Komaroff, L., Efstratiadis, A., Broome, S., Lomedico, P., Tizard, R., Naber, S. P., Chick, W. L., & Gilbert, W. (1978). A bacterial clone synthesizing proinsulin. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.75.8.3727
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
