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Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads.
Open accessAbstract
Evidence is presented that ColE1 hybrid plasmids carrying the cohesive-end site (cos) of lambda can be used as gene cloning vectors in conjunction with the lambda in vitro packaging system of Hohn and Murray [(1977) Proc. Natl. Acad. Sci. USA 74, 3259--3263]. Due to the requirement for a large DNA molecule for efficient packaging, there is a direct selection for hybrids carrying large sections of foreign DNA. The small vector plasmids do not contribute a large background in the transduced population, which is therefore markedly enriched for large hybrid plasmids (over 90%). The efficiency of the in vitro packaging system is on the order of 10(5) hybrid clones per microgram of foreign DNA for hybrids in the 20--30 million dalton range.
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Citations
Cited by
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts
Stewart, M. P., Langer, R., & Jensen, K. F. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00678 · Cited by 747
Metadata
- Authors
- Collins, J., & Hohn, B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1978-09
- Volume
- 75
- Issue
- 9
- Pages
- 4242-4246
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2094750135
- Cited by
- 356
- References
- 0
- FWCI
- 14.58
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads. https://app.cochat.ai/papers/10.1073/pnas.75.9.4242
- The paper
- Collins, J., & Hohn, B. (1978). Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.75.9.4242
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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