The DOI matches its Crossref record.
Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
Open accessAbstract
We describe a simple procedure for isolating specific mRNAs and for mapping them to the regions of the DNA from which they originate. The method involves hybridization of total cytoplasmic RNA to restriction fragments of DNA that have been fractionated in agarose gels and immobilized on nitrocellulose filters. The hybridization-selected RNAs are eluted and translated in a cell-free system in order to identify their encoded polypeptides. Optimal hybridization and filter washing conditions are given for selection of mRNAs from adenovirus 2-infected cells and transformed cells.
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Cited by
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Land, H., Schütz, G., Schmale, H., & Richter, D. (1982) · Nature · doi:10.1038/295299a0 · Cited by 569
Structure and evolution of the human involucrin gene
Eckert, R. L., & Green, H. (1986) · Cell · doi:10.1016/0092-8674(86)90884-6 · Cited by 391
Cloning of Complementary DNA for GAP-43, a Neuronal Growth-Related Protein
Karns, L. R., Ng, S.-C., Freeman, J. A., & Fishman, M. C. (1987) · Science · doi:10.1126/science.2437653 · Cited by 349
Two distinct classes of keratin genes and their evolutionary significance
FUCHS, E. (1981) · Cell · doi:10.1016/0092-8674(81)90362-7 · Cited by 305
Pancreatic preproglucagon cDNA contains two glucagon-related coding sequences arranged in tandem
Lund, P. K., Goodman, R. H., Dee, P. C., & Habener, J. F. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.2.345 · Cited by 295
Metadata
- Authors
- Ricciardi, R. P., Miller, J. S., & Roberts, B. E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1979-10
- Volume
- 76
- Issue
- 10
- Pages
- 4927-4931
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2066148522
- Cited by
- 486
- References
- 0
- FWCI
- 23.32
- Type
- journal article
- Language
- en
- Open access
- green
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- Ricciardi, R. P., Miller, J. S., & Roberts, B. E. (1979). Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.76.10.4927
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