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Amino acid sequence of bacteriorhodopsin.
Open accessAbstract
The complete primary structure of the purple membrane protein bacteriorhodopsin, which contains 248 amino acid residues, has been determined. Methods used for separation of the hydrophobic fragments included gel permeation and reverse-phase high-pressure liquid chromatography in organic solvents. The amino acid sequence was determined by a combination of automatic Edman degradation and mass spectrometric methods. The total sequence was derived by ordering of the CNBr fragments on the basis of methionine-containing peptides identified by gas chromatographic mass spectrometry and by analysis of N-bromosuccinimide fragments containing overlaps between CNBr fragments. The present sequence differs from that recently reported by Ovchinnikov and coworkers with respect to an additional tryptophan (position 138) and several amino acid assignments.
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Cited by
Microbial and Animal Rhodopsins: Structures, Functions, and Molecular Mechanisms
Ernst, O. P., Lodowski, D. T., Elstner, M., Hegemann, P., Brown, L. S., & Kandori, H. (2014) · Chemical Reviews · doi:10.1021/cr4003769 · Cited by 1,153
Electron-crystallographic Refinement of the Structure of Bacteriorhodopsin
Grigorieff, N., Ceska, T. A., Downing, K. H., Baldwin, J. M., & Henderson, R. (1996) · Journal of Molecular Biology · doi:10.1006/jmbi.1996.0328 · Cited by 956
Kubo, T., Fukuda, K., Mikami, A., Maeda, A., Takahashi, H., Mishina, M., Haga, T., Haga, K., Ichiyama, A., Kangawa, K., et al. (1986) · Nature · doi:10.1038/323411a0 · Cited by 906
A family of receptors coupled to guanine nucleotide regulatory proteins
Dohlman, H. G., Caron, M. G., & Lefkowitz, R. J. (1987) · Biochemistry · doi:10.1021/bi00384a001 · Cited by 631
Mass spectrometric determination of the amino acid sequence of peptides and proteins
Biemann, K., & Martin, S. A. (1987) · Mass Spectrometry Reviews · doi:10.1002/mas.1280060102 · Cited by 554
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Metadata
- Authors
- Khorana, H. G., Gerber, G. E., Herlihy, W. C., Gray, C. P., Anderegg, R. J., Nihei, K., & Biemann, K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1979-10
- Volume
- 76
- Issue
- 10
- Pages
- 5046-5050
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2143127913
- Cited by
- 412
- References
- 0
- FWCI
- 8.88
- Type
- journal article
- Language
- en
- Open access
- green
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- Khorana, H. G., Gerber, G. E., Herlihy, W. C., Gray, C. P., Anderegg, R. J., Nihei, K., & Biemann, K. (1979). Amino acid sequence of bacteriorhodopsin. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.76.10.5046
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