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Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood.
Open accessAbstract
A simple and rapid method for the determination of human T lymphocyte subclasses in buffy coat preparations or whole blood is described. This technique uses flow cytometry to distinguish lymphocytes from other leukocytes on the basis of their light-scattering properties. Lymnphocyte subclasses were enumerated by cellular immunofluorescence; the immunofluorescent signals were produced by monoclonal antibodies to surface differentiation antigens on T cells. Conventional techniques of enumerating T lymphocyte subclasses entail time-consuming (up to 2 days) density gradient and E rosette enrichement, and require at least 20 ml of blood. The method described here uses as little as 50 microliters of whole blood for each antibody tested and produces results within in 2 hr.
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Cited by
Jackson, M. R., Nilsson, T., & Peterson, P. A. (1990) · The EMBO Journal · doi:10.1002/j.1460-2075.1990.tb07513.x · Cited by 908
Impact of perceived self-efficacy in coping with stressors on components of the immune system.
Wiedenfeld, S. A., O'Leary, A., Bandura, A., Brown, S., Levine, S., & Raska, K. (1990) · Journal of Personality and Social Psychology · doi:10.1037/0022-3514.59.5.1082 · Cited by 262
Reversible Alterations in Immunoregulatory T Cells in Smoking
Miller, L. G., Goldstein, G., Murphy, M., & Ginns, L. C. (1982) · Chest · doi:10.1378/chest.82.5.526 · Cited by 260
Self-citationTerstappen, L. W. M. M., Meiners, H., & Loken, M. R. (1989) · Journal of Immunological Methods · doi:10.1016/0022-1759(89)90034-3 · Cited by 64
Metadata
- Authors
- Hoffman, R. A., Kung, P. C., Hansen, W. P., & Goldstein, G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1980-08
- Volume
- 77
- Issue
- 8
- Pages
- 4914-4917
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1993064155
- Cited by
- 445
- References
- 0
- FWCI
- 11.52
- Type
- journal article
- Language
- en
- Open access
- green
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- Hoffman, R. A., Kung, P. C., Hansen, W. P., & Goldstein, G. (1980). Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.77.8.4914
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