The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Efficient infection of monkey cells with DNA of simian virus 40.
Open accessAbstract
With standard protocols for DNA infection, only a small fraction (about 4%) of monkey cells exposed to purified DNA of simian virus 40 (SV40) exhibits signs of infection. We have devised a protocol by which we can extend the time of exposure of BSC-1 cells to DNA in the presence of low concentrations of DEAE-dextran. The efficiency of infection is proportional to the time of exposure. With an 8-hr exposure, we are reproducibly able to infect 25% of the cells, and we have been able to achieve levels of infection as high as 50% with a 16-hr exposure. The percentage of cells infected was measured either by scoring for nuclei positive for SV40 tumor antigen or by an infectious centers assay. We also report the use of ethidium bromide as a nonspecific nuclear counterstain in the immunofluorescence assay for SV40 tumor antigen.
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A new technique for the assay of infectivity of human adenovirus 5 DNA
Graham, F. L., & van der Eb, A. J. (1973) · Virology · doi:10.1016/0042-6822(73)90341-3 · Cited by 9,451
Selective extraction of polyoma DNA from infected mouse cell cultures
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Parker, B. A., & Stark, G. R. (1979) · Journal of Virology · doi:10.1128/jvi.31.2.360-369.1979 · Cited by 582
Interference in SV40 DNA infections: A possible basis for cellular competence
Wilson, J. H. (1978) · Virology · doi:10.1016/0042-6822(78)90385-9 · Cited by 30
Cited by
Thorens, B. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.18.8641 · Cited by 906
Comparative biochemical properties of normal and activated human ras p21 protein
McGrath, J. P., Capon, D. J., Goeddel, D. V., & Levinson, A. D. (1984) · Nature · doi:10.1038/310644a0 · Cited by 699
High efficiency polyoma DNA transfection of chloroquine treated cells
Luthman, H., & Magnusson, G. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.5.1295 · Cited by 677
The human interferon $alpha;/$beta; receptor: Characterization and molecular cloning
NOVICK, D. (1994) · Cell · doi:10.1016/0092-8674(94)90154-6 · Cited by 667
Cloning and expression of multiple protein kinase C cDNAs
Knopf, J. L., Lee, M.-H., Sultzman, L. A., Kriz, R. W., Loomis, C. R., Hewick, R. M., & Bell, R. M. (1986) · Cell · doi:10.1016/0092-8674(86)90874-3 · Cited by 658
Metadata
- Authors
- Sompayrac, L. M., & Danna, K. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1981-12
- Volume
- 78
- Issue
- 12
- Pages
- 7575-7578
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2084678798
- Cited by
- 391
- References
- 5
- FWCI
- 10.48
- Type
- journal article
- Language
- en
- Open access
- green
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- Sompayrac, L. M., & Danna, K. J. (1981). Efficient infection of monkey cells with DNA of simian virus 40. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.78.12.7575
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