The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Diphosphatidylglycerol is required for optimal activity of beef heart cytochrome c oxidase.
Open accessAbstract
Isolated beef heart cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1) contains four or five molecules of tightly bound diphosphatidylglycerol per monomer (2-heme complex). This lipid could be removed in part, or wholly, by mixing the enzyme with high concentrations of Triton X-100 and then centrifuging the mixture through a glycerol gradient equilibrated in the same detergent. Cytochrome c oxidase retaining three or more diphosphatidylglycerol molecules per monomer was fully active when assayed in 1-oleoyl lysophosphatidylcholine. Upon removal of one or more of these diphosphatidylglycerols, enzymic activity was lost. Full activation could be obtained by adding diphosphatidylglycerol to the assay mixture along with lysophosphatidylcholine but not by adding phosphatidylcholine or phosphatidylethanolamine. Direct binding experiments, kinetic studies, and previous work using arylazidocytochrome c derivatives [Bisson, R., Jacobs, B. & Capaldi, R. A. (1980) Biochemistry 10, 4173-4178], indicate that diphosphatidylglycerol is involved in binding of substrate cytochrome c to cytochrome c oxidase.
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Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Cited by
Raedschelders, K., Ansley, D. M., & Chen, D. D. Y. (2012) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2011.11.004 · Cited by 388
Phospholipid-Cytochrome c Interaction
Tuominen, E. K. J., Wallace, C. J. A., & Kinnunen, P. K. J. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m200056200 · Cited by 309
Kita, K., Konishi, K., & Anraku, Y. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)43304-7 · Cited by 274
Functional binding of cardiolipin to cytochromec oxidase
Robinson, N. C. (1993) · Journal of Bioenergetics and Biomembranes · doi:10.1007/bf00762857 · Cited by 265
Evidence for two distinct acidic phospholipid-binding sites in cytochrome c.
Rytömaa, M., & Kinnunen, P. K. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)42094-1 · Cited by 253
Metadata
- Authors
- Vik, S. B., Georgevich, G., & Capaldi, R. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1981-03
- Volume
- 78
- Issue
- 3
- Pages
- 1456-1460
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1992973572
- Cited by
- 169
- References
- 1
- FWCI
- 5.42
- Type
- journal article
- Language
- en
- Open access
- green
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- Vik, S. B., Georgevich, G., & Capaldi, R. A. (1981). Diphosphatidylglycerol is required for optimal activity of beef heart cytochrome c oxidase. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.78.3.1456
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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