The DOI matches its Crossref record.
Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal.
Open accessAbstract
Downstream placement of a strong transcriptional termination signal has made possible the cloning of bacteriophage T5 promoters known to exhibit high signal strength. The cloning system constructed contains two easily assayable indicator functions whose expression is controlled by the integration of promoters and terminators, respectively. By assessing transcription within the indicator regions, the efficiency of promoters as well as termination signals can be determined in vitro and in vivo.
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Citations
Cited by
Improved single and multicopy lac-based cloning vectors for protein and operon fusions
Simons, R. W., Houman, F., & Kleckner, N. (1987) · Gene · doi:10.1016/0378-1119(87)90095-3 · Cited by 1,518
Engineering BioBrick vectors from BioBrick parts
Shetty, R. P., Endy, D., & Knight, T. F. (2008) · Journal of Biological Engineering · doi:10.1186/1754-1611-2-5 · Cited by 760
Plasmid vectors for the selection of promoters
Brosius, J. (1984) · Gene · doi:10.1016/0378-1119(84)90136-7 · Cited by 423
Ward, J. M., Janssen, G. R., Kieser, T., Bibb, M. J., Buttner, M. J., & Bibb, M. J. (1986) · Molecular and General Genetics MGG · doi:10.1007/bf00422072 · Cited by 398
Stueber, D., & Bujard, H. (1982) · The EMBO Journal · doi:10.1002/j.1460-2075.1982.tb01329.x · Cited by 279
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Metadata
- Authors
- Gentz, R., Langner, A., Chang, A. C., Cohen, S. N., & Bujard, H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1981-08
- Volume
- 78
- Issue
- 8
- Pages
- 4936-4940
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2013189569
- Cited by
- 176
- References
- 0
- FWCI
- 10.51
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. https://app.cochat.ai/papers/10.1073/pnas.78.8.4936
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- Gentz, R., Langner, A., Chang, A. C., Cohen, S. N., & Bujard, H. (1981). Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.78.8.4936
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