The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
SOS chromotest, a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity.
Open accessAbstract
We present and evaluate the SOS chromotest, a bacterial test for detecting DNA-damaging agents. It is a colorimetric assay based on the induction by these agents of the SOS function sfiA, whose level of expression is monitored by means of a sfiA::lacZ operon fusion. The response is rapid (a few hours), and does not require survival of the tester strain. Dose-response curves for various chemicals include a linear region. The slope of this region is taken as a measure of the SOS inducing potency. Comparison for a number of substances of known genotoxicity of the SOS inducing potency determined in the SOS chromotest with the mutagenic potency determined in the Salmonella assay (mutatest) revealed a striking quantitative correlation over more than 7 orders of magnitude. The sensitivity of the SOS chromotest (lowest amount detected) is equal to that of the mutatest and generally 4-40 times higher than that of a phage induction assay (inductest). From a practical point of view our observations contribute to the validation of the SOS chromotest as a test for detecting genotoxins and in particular genotoxic carcinogens. From a theoretical standpoint the results suggest that mutagenic potency measured in the mutatest reflects the level of induction of an SOS function and that most genotoxins are inducers of the SOS response in bacteria.
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Citations
Cites
An inducible DNA replication–cell division coupling mechanism in E. coli
Huisman, O., & D'Ari, R. (1981) · Nature · doi:10.1038/290797a0 · Cited by 451
Self-citation
Cited by
Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli
Walker, G. C. (1984) · Microbiological Reviews · doi:10.1128/mmbr.48.1.60-93.1984 · Cited by 1,770
Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens
Oda, Y., Nakamura, S.-I., Oki, I., Kato, T., & Shinagawa, H. (1985) · Mutation Research/Environmental Mutagenesis and Related Subjects · doi:10.1016/0165-1161(85)90062-7 · Cited by 631
The SOS Chromotest, a colorimetric bacterial assay for genotoxins: procedures
Quillardet, P., & Hofnung, M. (1985) · Mutation Research/Environmental Mutagenesis and Related Subjects · doi:10.1016/0165-1161(85)90020-2 · Cited by 537
Self-citationMutagens in surface waters: a review
Ohe, T., Watanabe, T., & Wakabayashi, K. (2004) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2004.08.003 · Cited by 537
Where microbiology meets microengineering: design and applications of reporter bacteria
van der Meer, J. R., & Belkin, S. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2392 · Cited by 533
Metadata
- Authors
- Quillardet, P., Huisman, O., D'Ari, R., & Hofnung, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1982-10
- Volume
- 79
- Issue
- 19
- Pages
- 5971-5975
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2007749440
- Cited by
- 519
- References
- 1
- FWCI
- 8.90
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: SOS chromotest, a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity. https://app.cochat.ai/papers/10.1073/pnas.79.19.5971
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- Quillardet, P., Huisman, O., D'Ari, R., & Hofnung, M. (1982). SOS chromotest, a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.79.19.5971
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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