In vivo uptake of chylomicron [3H]retinyl ester by rat liver: evidence for retinol transfer from parenchymal to nonparenchymal cells.
Open accessAbstract
We have studied hepatic uptake of chylomicron retinyl ester. Chylomicrons were obtained from intestinal lymph of rats that were given retinol in groundnut oil by intraduodenal injection. When lymph was injected intravenously into normal rats, the radioactivity was cleared from blood with t1/2 approximately equal to 10 min. Retinyl ester was taken up initially by the liver, which, after 30 min, contained 80-90% of the radioactivity injected. Initially, most of the radioactivity was in hepatocytes, but after 30 min it disappeared from these cells and reappeared in nonparenchymal liver cells. After 2 hr these cells contained more radioactivity than the hepatocytes. When lymph was injected into vitamin A-deficient rats or rats given vitamin A in the form of retinoic acid, the plasma clearance and initial hepatic uptake of radioactivity were similar to that found in control animals. However, the nonparenchymal cells in these animals did not accumulate radioactivity. The current data suggest that vitamin A (in chylomicron remnants) is taken up initially by hepatocytes and then is released from these cells and delivered mainly to nonparenchymal liver cells in normal animals. In vitamin A-deficient rats, the vitamin is transferred from the hepatocytes to extrahepatic tissues.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Approximately 70% of chylomicrons are taken up by the liver, the main organ involved in storage of retinoids and regulation of serum vitamin A levels.
“Approximately 70% of chylomicrons are uptaken by the liver, the main organ involved in storage of retinoids and regulation of serum vitamin A levels [45,46].”
From Molecular Basis for Vitamin A Uptake and Storage in Vertebrates (2016) · cites this paper as [46]
The Abstract reports that rat liver contained 80–90% of injected chylomicron retinyl-ester radioactivity after 30 minutes, supporting substantial hepatic uptake of vitamin A but not the claim that approximately 70% of chylomicrons themselves are taken up. It describes transfer to nonparenchymal liver cells, but does not establish that the liver regulates serum vitamin A levels. (LLM, conf=0.88)
Experimental — accuracy not yet measuredThe possibility that fish oil enhances non-LPL-mediated clearance by tissues such as spleen, muscle, adipose tissue, or kidney cannot be excluded.
“The possibility that fish oil enhances non-LPL-mediated clearance by tissues such as spleen, muscle, adipose tissue, or kidney cannot be excluded, however (16,33).”
From Omega-3 fatty acid supplementation accelerates chylomicron triglyceride clearance (2003) · cites this paper as [33]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 31 · Cited by 190
Research works printed but not linked: 1
- Nilsson M., Berg T. Uptake and degradation of formaldehyde-treated 125I-labelled human serum albumin in rat liver cells in vivo and in vitro. Biochim Biophys Acta. 1977 Mar 29;497(1):171–182. doi: 10.1016/0304-4165(77)90150-7. [DOI] [PubMed] [Google Scholar] · DOI
- Authors
- Blomhoff, R., Helgerud, P., Rasmussen, M., Berg, T., & Norum, K. R.
- ORCID
- Rasmussen, M
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1982-12
- Volume
- 79
- Issue
- 23
- Pages
- 7326-7330
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC347332
- OpenAlex
- W2085284028
- Cited by
- 190
- References
- 31
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2022: 3 · 2021: 4 · 2020: 1 · 2019: 4 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 4 · 2014: 6 · 2013: 4 · 2012: 5
- FWCI
- 4.59
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Keywords
- hepatic uptake, non-parenchymal liver cells, hepatocytes, vitamin A deficiency, retinoic acid, chylomicron remnants, intestinal lymph, vitamin A distribution, rat liver
- MeSH terms
- Animals, Biological Transport, Cholesterol, Chylomicrons, Liver, Lymph, Male, Metabolic Clearance Rate, Tissue Distribution, Tretinoin, Triglycerides, Vitamin A, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
