The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Molecular cloning of human gastrin cDNA: evidence for evolution of gastrin by gene duplication.
Open accessAbstract
An oligo(dT)-primed cDNA copy of the mRNA coding for the human gastrin precursor was constructed from poly(A)-containing RNA from a human pancreatic, gastrin-producing tumor (a gastrinoma). The cDNA was inserted into the Pst I endonuclease site of plasmid pBR322 by the use of the poly(dC) and poly(dG) tailing procedure. Clones containing gastrin sequences were selected by hybridization to a purified single-stranded 32P-labeled gastrin cDNA probe. This probe was constructed with gastrinoma mRNA as template. As primer for the cDNA synthesis, we used a synthetic oligonucleotide mixture, d(AG-A-A-AG-T-C-C-A-T-C-C-A), corresponding to the gastrin-specific amino acid sequence Trp-Met-Asp-Phe. In this way we determined the nucleotide sequence of the entire coding region (303 nucleotides), the entire 3' untranslated region (102 nucleotides), and 8 nucleotides of the 5' untranslated region. A striking homology between parts of the coding region suggests that evolution of the gastrin gene has involved a gene duplication.
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Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
Sulfonation and molecular action
Strott, C. A. (2002) · Endocrine Reviews · doi:10.1210/er.2001-0040 · Cited by 487
Wiborg, O., Pedersen, M. S., Wind, A., Berglund, L. E., Marcker, K. A., & Vuust, J. (1985) · The EMBO Journal · doi:10.1002/j.1460-2075.1985.tb03693.x · Cited by 358
Self-citationThe New Biology of Gastrointestinal Hormones
REHFELD, J. F. (1998) · Physiological Reviews · doi:10.1152/physrev.1998.78.4.1087 · Cited by 329
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Metadata
- Authors
- Boel, E., Vuust, J., Norris, F., Norris, K., Wind, A., Rehfeld, J. F., & Marcker, K. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1983-05
- Volume
- 80
- Issue
- 10
- Pages
- 2866-2869
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1986288596
- Cited by
- 202
- References
- 1
- FWCI
- 6.74
- Type
- journal article
- Language
- en
- Open access
- green
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- Boel, E., Vuust, J., Norris, F., Norris, K., Wind, A., Rehfeld, J. F., & Marcker, K. A. (1983). Molecular cloning of human gastrin cDNA: evidence for evolution of gastrin by gene duplication. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.80.10.2866
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