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Isolation, primary structure, and synthesis of human hypothalamic somatocrinin: growth hormone-releasing factor.
Open accessAbstract
The hypophysiotropic peptide, growth hormone-releasing factor (GRF), was isolated from human hypothalamic-hypophysial tissues by means of acid extraction, immunoaffinity chromatography, gel filtration, and two steps of reverse-phase high-performance liquid chromatography. Amino acid sequence determination using a gas-phase sequencer and reverse-phase liquid chromatography of the native peptide and its synthetic replicates showed its primary structure to be as follows: H-Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln -Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Ser -Asn-Gln-Glu-Arg-Gly-Ala-Arg-Ala-Arg-Leu-NH2, which is identical to that of the GRF recently isolated and characterized from a human pancreatic tumor that had caused acromegaly. Human hypothalamic GRF shows major homologies (93%, 89%, and 86%, respectively) when its primary structure is compared to that of the hypothalamic GRF from the porcine, bovine, caprine, and ovine species.
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Cited by
Sonntag, W. E., Lynch, C. D., Cefalu, W. T., Ingram, R. L., Bennett, S. A., Thornton, P. L., & Khan, A. S. (1999) · The Journals of Gerontology Series A: Biological Sciences and Medical Sciences · doi:10.1093/gerona/54.12.b521 · Cited by 229
Hypothalamic Regulation of Anterior Pituitary Function
Ben-Shlomo, A., & Melmed, S. (2017) · The Pituitary · doi:10.1016/b978-0-12-804169-7.00002-7 · Cited by 11
Metadata
- Authors
- Ling, N., Esch, F., Böhlen, P., Brazeau, P., Wehrenberg, W. B., & Guillemin, R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1984-07
- Volume
- 81
- Issue
- 14
- Pages
- 4302-4306
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2058832065
- Cited by
- 291
- References
- 0
- FWCI
- 25.82
- Type
- journal article
- Language
- en
- Open access
- bronze
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- The paper
- Ling, N., Esch, F., Böhlen, P., Brazeau, P., Wehrenberg, W. B., & Guillemin, R. (1984). Isolation, primary structure, and synthesis of human hypothalamic somatocrinin: growth hormone-releasing factor. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.81.14.4302
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