The DOI matches its Crossref record.
Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.
Open accessAbstract
Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone alpha-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.
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Citations
Cited by
Shock and Tissue Injury Induced by Recombinant Human Cachectin
Tracey, K. J., Beutler, B., Lowry, S. F., Merryweather, J., Wolpe, S., Milsark, I. W., Hariri, R. J., Fahey, T. J., Zentella, A., Albert, J. D., et al. (1986) · Science · doi:10.1126/science.3764421 · Cited by 2,549
Self-citationDaly, R., & Hearn, M. T. W. (2005) · Journal of Molecular Recognition · doi:10.1002/jmr.687 · Cited by 746
Clare, J. J., Romanes, M. A., Rayment, F. B., Rowedder, J. E., Smith, M. A., Payne, M. M., Sreekrishna, K., & Henwood, C. A. (1991) · Gene · doi:10.1016/0378-1119(91)90152-2 · Cited by 329
Protein Localization and Membrane Traffic in Yeast
Schekman, R. (1985) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.01.110185.000555 · Cited by 266
Laboratory Evolution of High-Redox Potential Laccases
Maté, D., García-Burgos, C., García-Ruiz, E., Ballesteros, A. O., Camarero, S., & Alcalde, M. (2010) · Chemistry & Biology · doi:10.1016/j.chembiol.2010.07.010 · Cited by 166
Metadata
- Authors
- Brake, A. J., Merryweather, J. P., Coit, D. G., Heberlein, U. A., Masiarz, F. R., Mullenbach, G. T., Urdea, M. S., Valenzuela, P., & Barr, P. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1984-08
- Volume
- 81
- Issue
- 15
- Pages
- 4642-4646
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2066837313
- Cited by
- 485
- References
- 0
- FWCI
- 10.33
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. https://app.cochat.ai/papers/10.1073/pnas.81.15.4642
- The paper
- Brake, A. J., Merryweather, J. P., Coit, D. G., Heberlein, U. A., Masiarz, F. R., Mullenbach, G. T., Urdea, M. S., Valenzuela, P., & Barr, P. J. (1984). Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.81.15.4642
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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