The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Antigen presentation by supported planar membranes containing affinity-purified I-Ad.
Open accessAbstract
I-Ad, purified from A20-1.11 cells by affinity chromatography, was incorporated into supported planar membranes by incubation of I-Ad-containing phospholipid vesicles with clean glass coverslips. Such planar membranes present a peptide digest of ovalbumin to the ovalbumin-specific, I-Ad-restricted T-cell hybridoma 3DO-54.8, resulting in the antigen-specific release of interleukin 2. However, when the same material was provided in the form of small unilamellar vesicles, no response was obtained. Antigen presentation by the I-Ad-containing planar membranes was inhibited by the monoclonal antibody MKD6 (anti-I-Ad) but not by the antibody 10-2.16 (anti-I-Ak). The antibody GK1.5, which recognizes the T-cell surface antigen L3T4, was also inhibitory. In contrast to the results with purified I-Ad, crude membrane preparations from A20-1.11 cells were effective in antigen presentation in both planar and vesicular forms.
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Cites
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Cited by
Sequence analysis of peptides bound to MHC class II molecules
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Metadata
- Authors
- Watts, T. H., Brian, A. A., Kappler, J. W., Marrack, P., & McConnell, H. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1984-12
- Volume
- 81
- Issue
- 23
- Pages
- 7564-7568
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2017428346
- Cited by
- 274
- References
- 18
- FWCI
- 8.40
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Watts, T. H., Brian, A. A., Kappler, J. W., Marrack, P., & McConnell, H. M. (1984). Antigen presentation by supported planar membranes containing affinity-purified I-Ad. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.81.23.7564
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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